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You are here:Home » Antibodies » Abs to Oncoproteins » Anti -c-Abl (ABL1, v-abl, Abelson Murine Leukemia Viral Oncogene Homolog 1, ABL, c-ABL, JTK7, p150, Proto-Oncogene Tyrosine-Protein Kanase ABL1, v-abl)

Anti -c-Abl (ABL1, v-abl, Abelson Murine Leukemia Viral Oncogene Homolog 1, ABL, c-ABL,
JTK7, p150, Proto-Oncogene Tyrosine-Protein Kanase ABL1, v-abl)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IH IF
Bcr-Abl hybrid gene product is a 210kD protein known to be assembled in K562 (human chronic myelogenous leukemia) cell line. c-Abl proto-oncogene encodes a nonreceptor type protein tyrosine kinase that is ubiquitously expressed and highly conserved in metazoan evolution. c-Abl protein is distributed in both nucleus and cytoplasm of cells. In chronic myelogenous leukemia and in a subset of acute lymphoblastic leukemias, c-abl proto-oncogene undergoes a (9;22) chromosomal translocation producing a novel rearranged chromosome (the Philadelphia chromosome) as the result of the fusion of c-Abl sequences from chromosome 9 to Bcr gene on chromosome 22. The molecular consequence of translocation is generation of a chimeric Bcr/Abl mRNA encoding activated Abl protein tyrosine kinase. It has been implicated in regulation of cell proliferation, differentiation, apoptosis, cell adhesion and stress response (1-3). c-Abl kinase activity is increased in vivo by diverse physiological stimuli including integrin activation, PDGF stimulation and binding to c-Jun, Nck and RFX1. The in vivo mechanism of regulation of c-Abl kinase activity is not completely understood. Tyrosine 245 is located in the linker region between the SH2 and catalytic domains, which is conserved among the Abl family. Phosphorylation of Tyr245 is involved in activation of c-Abl kinase activity (5). Tyrosine 412 is located in the kinase activation loop of c-Abl molecules; phosphorylation of this residue is required for the kinase activity (6). Thr735 is in a conserved 14-3-3 protein binding motif, which can be phosphorylated upon stress stimulation or TPA treatment (Wu, J. et al. unpublished data). Phosphorylation of Thr735 in c-Abl may play an important role in regulation of c-Abl localization as well as its function.
Applications:
Suitable for use in Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications have not been tested.
Catalog #C0013-02A
Recommended DilutionImmunoprecipitation: 2ug/mg protein lysate (Use Protein G).
Western Blot: 1ug/ml for 2hrs at RT
Immunohistochemistry (Formalin/paraffin): 2-4ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 8 for 10-20 minutes followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by the researcher.
Positive Control HeLa cells or placenta
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,g
Clone No2Q217
HostMouse
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also supplied without BSA and sodium azide. See C0013-02AX.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant Abl protein. Cellular Localization: Cytoplasmic and nuclear
EpitopeSH2 domain. MW of Antigen: 120kD.
SpecificityRecognizes human c-Abl p120 and chimeric Bcr/Abl proteins.. Species crossreactivity: mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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