| c-erbB-2 is a membrane-associated oncoprotein having considerable sequence homology to EGFR. C-erbB-2 is the second member of the EGF receptor tyrosine kinase family of growth factor receptors. Overexpression of c-erbB-2/HER-2 is reportedly a marker of poor prognosis in node-positive and in defined subsets of node-negative breast cancer. Elevated levels of c-erbB-2/HER-2 has also been implicated in response to chemotherapy. |
|
| Catalog # | C0015X1 |
| Applications | Suitable for ELISA, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications have not been tested. |
| Recommended Dilution | Immunoprecipitation: 10ug/mg protein lysate (Use Protein A). |
| Western Blot: 5-10ug/ml for 2 hours at RT. |
| Immunohistochemistry (Formalin/paraffin): 2.5-5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. |
| Optimal dilutions to be determined by the researcher. |
| Positive Control | SKBR-3 cells, T47D cells or breast carcinomas. |
| Control Peptide | C0015-30: c-erbB2, aa1243-1255, Control Peptide (HER2, neu Receptor) |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.4. No stablilizing proteins and no preservatives added. Available with BSA and sodium azide. See C0015. Also available without BSA and sodium azide. See C0015X. |
| Purity | Purified by Protein A affinity chromatography. |
| Immunogen | Synthetic peptide aa1243-1255 (AENPEYLGLDVPV) derived from C-terminus of human c-erbB-2/HER-2 protein (KLH coupled). Sequence is identical in rat neu protein. Cellular Localization |
| Specificity | Recognizes wild and mutant (oncogenic) forms of human c-erbB-2 protein (185kD). Does not crossreact with c-erbB-11 (EGF-R), c-erbB-3, or c-erbB-4. Species Crossreactivity: monkey, rat and mouse. |
|
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
