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You are here:Home » Antibodies » Abs to Oncoproteins » Anti -c-erbB2, p185 (HER2, neu Receptor) (Glycerol, BSA & Azide Free)

Anti -c-erbB2, p185 (HER2, neu Receptor) (Glycerol, BSA & Azide Free)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IP IH
c-erbB-2 is a membrane-associated oncoprotein having considerable sequence homology to EGFR. C-erbB-2 is the second member of the EGF receptor tyrosine kinase family of growth factor receptors. Overexpression of c-erbB-2/HER-2 is reportedly a marker of poor prognosis in node-positive and in defined subsets of node-negative breast cancer. Elevated levels of c-erbB-2/HER-2 has also been implicated in response to chemotherapy.
Catalog #C0015X1
ApplicationsSuitable for ELISA, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionImmunoprecipitation: 10ug/mg protein lysate (Use Protein A).
Western Blot: 5-10ug/ml for 2 hours at RT.
Immunohistochemistry (Formalin/paraffin): 2.5-5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by the researcher.
Positive ControlSKBR-3 cells, T47D cells or breast carcinomas.
Control PeptideC0015-30: c-erbB2, aa1243-1255, Control Peptide (HER2, neu Receptor)
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4. No stablilizing proteins and no preservatives added. Available with BSA and sodium azide. See C0015. Also available without BSA and sodium azide. See C0015X.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide aa1243-1255 (AENPEYLGLDVPV) derived from C-terminus of human c-erbB-2/HER-2 protein (KLH coupled). Sequence is identical in rat neu protein. Cellular Localization
SpecificityRecognizes wild and mutant (oncogenic) forms of human c-erbB-2 protein (185kD). Does not crossreact with c-erbB-11 (EGF-R), c-erbB-3, or c-erbB-4. Species Crossreactivity: monkey, rat and mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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