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Anti -C/EBPb (NT) (CCAAT/Enhancer-Binding Protein beta, NFIL6)
Anti -C/EBPb (NT) (CCAAT/Enhancer-Binding Protein beta, NFIL6)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rabbit |
Supernatant |
B IP FC |
|
| The transcription factor CCAAT/Enhancer Binding Protein beta (C/EBPb, also known as NFIL6) is a highly conserved B-Zip family member. C/EBPb regulates multiple genes involved in hepatic function, immune function and adipogenesis, and its functions are generally distinct from those of other C/EBP family members (alpha, gamma, delta, epsilon). C/EBPb is present in cells as three alternate translation products: two transcription activating forms known as LAP1 and LAP2 (~35 and 32kD in rodents, ~43kD and 40kD in humans) and a truncated inhibitory form known as LIP (~20kD) which lacks the N-terminal transcription activation domain. C/EBP beta is phosphorylated at multiple sites which are determinants of some of its activities (e.g. activation of c-fos, adipogenesis). A recent report shows that it is acetylated at Lys215/216, and others report that it can be sumoylated. C/EBPb is also acetylated at Lys39 in the transcription activation domain, and mutation of Lys39 impairs its ability to activate transcription of multiple genes, including several involved in adipogenesis. | | | Catalog # | C0022-10A | | Applications | Suitable for use in Flow Cytometry, Western Blot, and Immunoprecipitation. Other applications not tested. | | Recommended Dilution | Flow Cytometry: 1:20 | | Western Blot: 1:2000 | | Immunoprecipitation: 1:50 | | Optimal dilutions to be determined by the researcher. | | Positive Control | PC-12 cell lysate | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG | | Clone No | 9i149 | | Host | Rabbit | | Source | Rat | | Concentration | Not determined | | Form | Supplied as a liquid in Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol. | | Purity | Supernatant | | Immunogen | Synthetic peptide corresponding to residues in N-terminus of rat full-length C/EBPb. | | Specificity | Recognizes the LAP form of rat C/EBPb. Species Crossreactivity: mouse. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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