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You are here:Home » Antibodies » Abs to Binding Proteins » Anti -C/EBPb (NT) (CCAAT/Enhancer-Binding Protein beta, NFIL6)

Anti -C/EBPb (NT) (CCAAT/Enhancer-Binding Protein beta, NFIL6)


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Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IP FC
The transcription factor CCAAT/Enhancer Binding Protein beta (C/EBPb, also known as NFIL6) is a highly conserved B-Zip family member. C/EBPb regulates multiple genes involved in hepatic function, immune function and adipogenesis, and its functions are generally distinct from those of other C/EBP family members (alpha, gamma, delta, epsilon). C/EBPb is present in cells as three alternate translation products: two transcription activating forms known as LAP1 and LAP2 (~35 and 32kD in rodents, ~43kD and 40kD in humans) and a truncated inhibitory form known as LIP (~20kD) which lacks the N-terminal transcription activation domain. C/EBP beta is phosphorylated at multiple sites which are determinants of some of its activities (e.g. activation of c-fos, adipogenesis). A recent report shows that it is acetylated at Lys215/216, and others report that it can be sumoylated. C/EBPb is also acetylated at Lys39 in the transcription activation domain, and mutation of Lys39 impairs its ability to activate transcription of multiple genes, including several involved in adipogenesis.
Catalog #C0022-10A
ApplicationsSuitable for use in Flow Cytometry, Western Blot, and Immunoprecipitation. Other applications not tested.
Recommended DilutionFlow Cytometry: 1:20
Western Blot: 1:2000
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.
Positive ControlPC-12 cell lysate
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No9i149
ConcentrationNot determined
FormSupplied as a liquid in Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol.
ImmunogenSynthetic peptide corresponding to residues in N-terminus of rat full-length C/EBPb.
SpecificityRecognizes the LAP form of rat C/EBPb. Species Crossreactivity: mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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