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You are here:Home » Antibodies » Abs to Oncoproteins » Anti -c-erbB2 (HER-2, neu Receptor)

Anti -c-erbB2 (HER-2, neu Receptor)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP IH FC
c-erbB-2 is the cellular homolog of the neu oncogene. c-erbB-2 is comprised of an extracellular ligand binding domain, a single transmembrane domain, a tyrosine kinase domain, and a C terminal region which mediates signaling. c-erbB-2 forms heterodimers with other members of the EGF receptor family, such as c-erbB-1. In response to ligand binding, c-erbB-2 is autophosphorylated on five tyrosine residues at its C terminus, producing docking sites for SH2-domain containing proteins such as phospholipase C , PI3-K, Src, CHK, and others. Over-expression of c-erbB-2 is observed in 15-40% of primary human breast tumors, and correlates with poor prognosis in node positive breast cancer. c-erbB-2 over-expression is also observed in ovarian, gastric, salivary, and non-small cell lung carcinomas.
Catalog #C0026-11N1
ApplicationsSuitable for use in Immunoprecipitation, Western Blot, ELISA, Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunoprecipitation (native and denatured): 2ug/mg cell lysate
Western Blot: 1-2ug/ml with 2 hour incubation
Immunohistochemistry (Formalin fixed paraffin embedded): 2-4ug/ml with 30 minute incubation. Requires boiling the sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes, cool at RT for 10 minutes.
Optimal dilutions to be determined by the researcher.
Positive ControlSKBR-3 cells (breast cancer cell line), T47D cells.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No5J297
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4. Preservative free.
PurityPurified by Protein G affinity chromatography.
ImmunogenCytoplasmic domain of human c-erbB-2 protein. Cellular Localization: Cell membrane.
SpecificityRecognizes human c-erbB-2, a receptor protein tyrosine kinase with Mr=185kD, which is also known as p185HER-2. Species Crossreactivity: mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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