| c-erbB-2 is the cellular homolog of the neu oncogene. c-erbB-2 is comprised of an extracellular ligand binding domain, a single transmembrane domain, a tyrosine kinase domain, and a C terminal region which mediates signaling. c-erbB-2 forms heterodimers with other members of the EGF receptor family, such as c-erbB-1. In response to ligand binding, c-erbB-2 is autophosphorylated on five tyrosine residues at its C terminus, producing docking sites for SH2-domain containing proteins such as phospholipase C , PI3-K, Src, CHK, and others. Over-expression of c-erbB-2 is observed in 15-40% of primary human breast tumors, and correlates with poor prognosis in node positive breast cancer. c-erbB-2 over-expression is also observed in ovarian, gastric, salivary, and non-small cell lung carcinomas. |
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| Catalog # | C0026-11N1 |
| Applications | Suitable for use in Immunoprecipitation, Western Blot, ELISA, Flow Cytometry and Immunohistochemistry. Other applications not tested. |
| Recommended Dilution | Immunoprecipitation (native and denatured): 2ug/mg cell lysate |
| Western Blot: 1-2ug/ml with 2 hour incubation |
| Immunohistochemistry (Formalin fixed paraffin embedded): 2-4ug/ml with 30 minute incubation. Requires boiling the sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes, cool at RT for 10 minutes. |
| Optimal dilutions to be determined by the researcher. |
| Positive Control | SKBR-3 cells (breast cancer cell line), T47D cells. |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 5J297 |
| Host | Mouse |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.4. Preservative free. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | Cytoplasmic domain of human c-erbB-2 protein. Cellular Localization: Cell membrane. |
| Specificity | Recognizes human c-erbB-2, a receptor protein tyrosine kinase with Mr=185kD, which is also known as p185HER-2. Species Crossreactivity: mouse and rat. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
