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You are here:Home » Molecular Biology » MB-Protein Kinases » C-TAK1, Recombinant, Human (Cdc25C-associated Protein Kinase 1, cTAK1, MAP/microtubule Affinity-regulating Kinase 3, MARK3, ELKL Motif Kinase 2, EMK2, EMK-2, KP78, PAR1A, Par-1a, Protein Kinase STK10, Ser/Thr Protein Kinase PAR-1, Serine/threonine-protein

C-TAK1, Recombinant, Human (Cdc25C-associated Protein Kinase 1, cTAK1, MAP/microtubule
Affinity-regulating Kinase 3, MARK3, ELKL Motif Kinase 2, EMK2, EMK-2, KP78, PAR1A,
Par-1a, Protein Kinase STK10, Ser/Thr Protein Kinase PAR-1, Serine/threonine-protein

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Specifications

Cdc25C-associated kinase 1 (C-TAK1) has been implicated in cell cycle regulation and Ras signaling through its interactions with two putative substrates, the Cdc25C phosphatase and the MAPK scaffold KSR1.
Catalog #C0040
Source  N-terminal, His-tagged fusion protein corresponding to human c-TAK1, expressed in E. Coli
ApplicationsKinase Assay:489U/mg
Optimal dilutions to be determined by the researcher.
Storage and StabilityFor long-term storage, store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight90kD
SourceE. coli
Purity85% by SDS-PAGE. Purified by Nickel-NTA agarose chromatography
Concentration~0.1mg/ml
FormSupplied as a liquid in 50mM HEPES, pH 7.5, 200mM sodium chloride, 5mM DTT, 0.03% Brij-35, 50% glycerol.
Specific Activity489U/mg. 1U is equal to 1nmole of phosphate transferred to CHKtide substrate peptide (KKKVSRSGLYRSPSM-PENLNRPR) per minute at 30ºC with a final ATP concentration of 100uM.
Important NoteThis product is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Alternate namesEC=2.7.11.1


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