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Anti -C4.4A (LYPD3, Y6/PLAUR domain containing 3 GPI-anchored metastasis-associated protein C4.4A homolog, Ly6/PLAUR domain-containing protein 3 precursor, Matrigel-induced gene C4 protein, MIG-C4, UNQ491/PRO1007)
Anti -C4.4A (LYPD3, Y6/PLAUR domain containing 3 GPI-anchored metastasis-associated
protein C4.4A homolog, Ly6/PLAUR domain-containing protein 3 precursor, Matrigel-induced
gene C4 protein, MIG-C4, UNQ491/PRO1007)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Sheep |
Affinity Purified |
E B |
|
| LYPD3 (Ly6/PLAUR-domain containing protein 3; also MIG-C4 and GPI-anchored metastasis-associated protein C4.4A) is an 85-95kD, variably glycosylated member of the Ly6 family of GPI-anchored proteins. It is expressed on transitional epithelium, keratinocytes and monocytes, and binds laminin 1 and 5, plus Galectin-3. LYPD3 is believed to support cell migration. Mature human LYPD3 is a 296aa GPI-linked glycoprotein. It contains two UPAR domains (aa33-126 and 140-222), and an ADAM cleavage site (aa312-325). The molecule shows extensive N- and O-linked glycosylation. There is one potential isoform that shows a deletion of aa129-180. Over aa 31-286, human LYPD3 shares 81% aa identity with mouse LYPD3. | | | | | | Applications: | | | Suitable for use in ELISA and Western Blot. Other applications not tested. | | | Catalog # | C0045-75 | | Recommended Dilution | ELISA (direct): 0.5-1.0ug/ml with the appropriate secondary reagents to detect human C4.4A. The detection limit for rhC4.4A is approximately 0.2ng/well. | | Western Blot: 1.0ug/ml. Tissue lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 1ug/ml sheep anti-hC4.4A. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Sheep | | Source | Human | | Concentration | ~0.2mg/ml | | Form | Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Recombinant corresponding to aa 31-286 human C4.4A expressed in NSO cells (O95274). | | Specificity | Recognizes human C4.4A. In direct ELISA, shows ~15% cross-reactivity with rmC4.4A. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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