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You are here:Home » Antibodies » Abs to Carbohydrates, Glycoproteins » Anti -C4.4A (LYPD3, Y6/PLAUR domain containing 3 GPI-anchored metastasis-associated protein C4.4A homolog, Ly6/PLAUR domain-containing protein 3 precursor, Matrigel-induced gene C4 protein, MIG-C4, UNQ491/PRO1007)

Anti -C4.4A (LYPD3, Y6/PLAUR domain containing 3 GPI-anchored metastasis-associated
protein C4.4A homolog, Ly6/PLAUR domain-containing protein 3 precursor, Matrigel-induced
gene C4 protein, MIG-C4, UNQ491/PRO1007)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Affinity Purified E B
LYPD3 (Ly6/PLAUR-domain containing protein 3; also MIG-C4 and GPI-anchored metastasis-associated protein C4.4A) is an 85-95kD, variably glycosylated member of the Ly6 family of GPI-anchored proteins. It is expressed on transitional epithelium, keratinocytes and monocytes, and binds laminin 1 and 5, plus Galectin-3. LYPD3 is believed to support cell migration. Mature human LYPD3 is a 296aa GPI-linked glycoprotein. It contains two UPAR domains (aa33-126 and 140-222), and an ADAM cleavage site (aa312-325). The molecule shows extensive N- and O-linked glycosylation. There is one potential isoform that shows a deletion of aa129-180. Over aa 31-286, human LYPD3 shares 81% aa identity with mouse LYPD3.
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Catalog #C0045-75
Recommended DilutionELISA (direct): 0.5-1.0ug/ml with the appropriate secondary reagents to detect human C4.4A. The detection limit for rhC4.4A is approximately 0.2ng/well.
Western Blot: 1.0ug/ml. Tissue lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 1ug/ml sheep anti-hC4.4A.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
PurityPurified by immunoaffinity chromatography.
ImmunogenRecombinant corresponding to aa 31-286 human C4.4A expressed in NSO cells (O95274).
SpecificityRecognizes human C4.4A. In direct ELISA, shows ~15% cross-reactivity with rmC4.4A.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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