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You are here:Home » Antibodies » Abs to Carbohydrates, Glycoproteins » Anti -C4.4A (LYPD3, Y6/PLAUR domain containing 3 GPI-anchored metastasis-associated protein C4.4A homolog, Ly6/PLAUR domain-containing protein 3 precursor, Matrigel-induced gene C4 protein, MIG-C4, UNQ491/PRO1007)

Anti -C4.4A (LYPD3, Y6/PLAUR domain containing 3 GPI-anchored metastasis-associated
protein C4.4A homolog, Ly6/PLAUR domain-containing protein 3 precursor, Matrigel-induced
gene C4 protein, MIG-C4, UNQ491/PRO1007)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH
C4.4A (Ly6/PLAUR-domain containing protein 3; also MIG-C4 and GPI-anchored metastasis-associated protein C4.4A) is an 85-95kD, variably glycosylated member of the Ly6 family of GPI-anchored proteins. It is expressed on transitional epithelium, keratinocytes and monocytes, and binds laminin 1 and 5, plus Galectin-3. C4.4A is believed to support cell migration.
Catalog #C0045-75B
ApplicationsSuitable for Western Blot, Immunohistochemistry and ELISA. Other applicatons not tested.
Recommended DilutionWestern Blot: 1ug/ml. Tissue lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted.
Immunohistochemistry: 2-15ug/ml will detect C4.4A in cells and tissues.
Direct ELISA: 0.5-1ug/ml. The detection limit is ~0.3ng/well.
Optimal dilutions should be determined by each laboratory for each application.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceMouse
Concentration~0.2mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
PurityPurified by immunoaffinity chromatography.
ImmunogenRecombinant corresponding to mouse C4.4A expressed in NS0 cells (Q91YK8).
SpecificityRecognizes mouse C4.4A. In direct ELISA, this antibody shows approximately 40% cross-reactivity with rhC4.4A/LYPD3.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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