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You are here:Home » Antibodies » Abs to Cancer Markers » Anti -CA125 Cancer Antigen (Ovarian Cancer)

Anti -CA125 Cancer Antigen (Ovarian Cancer)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified IH
MUC16/CA125 antigen is a serum marker that is used routinely in gynecologic practice to monitor patients with ovarian cancer. It is a mullerian duct differentiation antigen that is overexpressed in epithelial ovarian cancer cells and secreted into the blood, although its expression is not entirely confined to ovarian cancer. Most biochemical studies have concluded that MUC16/CA125 is a high molecular mass glycoprotein.
Catalog #C0050-11A
According to the ISOBM-TD-1 workshop, CA125 contains three antigenic domains A-domain (Oc125), B-domain (M11), C-Domain (Ov197). The CA-125 antigen is present on a mucin-like glycoprotein of >200kD and is detectable on the apical surface of normal endometrium and endocervix.
ApplicationsSuitable for use in Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry (Paraffin-embedded sections): Requires epitope retrieval using citrate buffer. Enzyme digestion not required.
Optimal dilutions to be determined by the researcher.
Positive Control Ovarian carcinoma/adenocarcinoma.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3G33
HostMouse
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in PBS, pH 7.4, 1% BSA, 0.1% sodium azide, before the addition of glycerol to 40%.
PurityPurified
ImmunogenOvarian cancer cell line OVCA433 derived from patient with serous papillary cystadenocarcinoma.
Expected Staining PatternMmebrane
SpecificityRecognizes the human ovarian cancer-associated antigen 125 (CA 125 antigen).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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