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You are here:Home » Antibodies » Abs to Cancer Markers » Anti -CA125 Cancer Antigen, A Domain (Ovarian Cancer)

Anti -CA125 Cancer Antigen, A Domain (Ovarian Cancer)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
MUC16/CA125 antigen is a serum marker that is used routinely in gynecologic practice to monitor patients with ovarian cancer. It is a mullerian duct differentiation antigen that is overexpressed in epithelial ovarian cancer cells and secreted into the blood, although its expression is not entirely confined to ovarian cancer. Most biochemical studies have concluded that MUC16/CA125 is a high molecular mass glycoprotein.
Catalog #C0050-13
According to the ISOBM-TD-1 workshop, CA125 contains three antigenic domains A-domain (Oc125), B-domain (M11), C-Domain (Ov197). The CA-125 antigen is present on a mucin-like glycoprotein of >200kD and is detectable on the apical surface of normal endometrium and endocervix.
ApplicationsSuitable for use in ELISA and Western Blot. HRP conjugation is not recommended. Other applications have not been tested.
Recommended DilutionsOptimal dilutions to be determined by the researcher.
Recommended PairCA125 A domain
capture: C0050-13
detection: C0050-16
CA125 B Domain capture C0050-15
detection: C0050-13
HydridomaSp2/0 myeloma cells with spleen cells from Balb/c mice.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No1.B.827
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 10mM Tris, 150mM sodium chloride, pH 7.5, 0.05% sodium azide.
PurityPurified by ion exchange chromatography.
ImmunogenCA125 antigen purified from human ovarian carcinoma
(MW>1million Daltons)
SpecificityRecognizes A-domain (Oc125-like) in the protein core of the CA125 ovarian cancer antigen.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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