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You are here:Home » Antibodies » Antibodies-Cancer Markers » Anti -CA15-3 Cancer Antigen (Breast Cancer)

Anti -CA15-3 Cancer Antigen (Breast Cancer)


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Clone Host Grade Applications
Monoclonal Mouse Purified IH
The DF3 antigen is present in high levels on apical borders of differentiated secretory mammary epithelial cells and in the cytosol of less differentiated cells. Malignant tumors display cytoplasmic expression while in benign tumors, expression is seen on apical surfaces.
Catalog #C0050-32B
ApplicationSuitable for use in Immunohistochemistry. Other applications have not been tested.
Recommended DilutionsImmunohistochemistry (Formalin/paraffin): 1:40 for biotin based detection systems. Pre-treatment is not required. For optimal staining, primary antibody should be incubated 20-60 minutes at RT.
Optimal dilutions to be determined by researcher.
Positive ControlInfiltrating ductal human breast carcinoma.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap
Clone TypeMonoclonal
Clone No6D184
ConcentrationNot Determined
FormSupplied as a liquid in PBS, pH 7.2, BSA, 0.1% sodium azide
ImmunogenMembrane-enriched fraction of a human carcinoma metastatic to the liver.
SpecificityRecognizes human DF3 antigenic determinant (290kD), a portion of a 20aa tandemly repeated sequence present on the core protein of an epithelial cell-associated mucin (MAM-6). Reacts with a large proportion of primary breast carcinomas and breast tumors metastatic to axillary lymph nodes or distant sites, and with 95% of benign, borderline and malignant tumors of the ovary. Reactivity with lung and colorectal carcinomas has also been demonstrated.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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