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You are here:Home » Antibodies » Antibodies-Cancer Markers » Anti -CA50, Cancer Antigen (Colorectal Carcinoma Cell Line, COLO-205)

Anti -CA50, Cancer Antigen (Colorectal Carcinoma Cell Line, COLO-205)


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E
The CRC hybridoma cell line recognizes human colorectal carcinoma antigen (CRC). It was transfected by mpSV2gpt. The JXT3 hybridoma cell line recognizes CD3 molecule on human T cells. it was transfected by mpSV2neo. The production of quadroma CRCgpt/CD3neo included a conventinal somatic fusion between CRC and JXT3 cells. These hybridomas were selected and cloned in the media containing both mycophenolic acid and geneticin. Quadromas showing both murine IgG1 and IgG2a were selected and subcloned for the purification of hybrid bispecific antibody CRC/CD3.
Catalog #C0085-01A
ApplicationsSuitable for use in ELISA. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No3H76
FormSupplied as a liquid in PBS, pH 7.0, 40% glycerol.
PurityPurified by Protein G affinity chromatography.
ImmunogenHuman colorectal carcinoma antigen (CRC)/ CD3 molecule on human T cells
SpecificityRecognizes human colorectal carcinoma antigen (CRC)/ CD3 molecule on human T cells.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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