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You are here:Home » Antibodies » Abs to Cancer Markers » Anti -CA50, Cancer Antigen (Colorectal Carcinoma Cell Line, COLO-205)

Anti -CA50, Cancer Antigen (Colorectal Carcinoma Cell Line, COLO-205)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E
The CRC hybridoma cell line recognizes human colorectal carcinoma antigen (CRC). It was transfected by mpSV2gpt. The JXT3 hybridoma cell line recognizes CD3 molecule on human T cells. it was transfected by mpSV2neo. The production of quadroma CRCgpt/CD3neo included a conventinal somatic fusion between CRC and JXT3 cells. These hybridomas were selected and cloned in the media containing both mycophenolic acid and geneticin. Quadromas showing both murine IgG1 and IgG2a were selected and subcloned for the purification of hybrid bispecific antibody CRC/CD3.
Catalog #C0085-01E
ApplicationsSuitable for use in ELISA. Other applications not tested.
Recommended DilutionELISA: 0.2ng/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG3,k
Clone No12K257
HostMouse
SourceHuman
ConcentrationNot determined
FormSupplied as a lyophilized powder from 0.01M PBS, pH 7.2. Reconstitute in ddH2O to 1mg/ml.
PurityPurified by Protein G affinity chromatography.
ImmunogenHuman CA 50 protein.
SpecificityRecognizes human CA 50.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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