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You are here:Home » Antibodies » Abs to Apoptosis, Caspase » Anti -CAD (CT) (Caspase Activated Deoxyribonuclease)

Anti -CAD (CT) (Caspase Activated Deoxyribonuclease)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD (for caspase activated deoxyribonuclease) (1,2). The human homologue of mouse CAD was more recently identified by two groups independently and termed CPAN and DFF40 (3,4). Human DFF45 and its mouse homologue ICAD are the inhibitors of CPAN/DFF40 and CAD, respectively (1, 2, 5). Upon cleavage of DFF45/ICAD by activated caspase, DFF40/CAD is released and activated and eventually causes the degradation of DNA in the nuclei (1-4). Activation of CAD/DFF40, which causes DNA degradation, is the hallmark of apoptotic cell death.
Catalog #C0105
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionsWestern Blot: 1:250-1:500.
Optimal dilutions to be determined by researcher.
Positive ControlTissue lysate (25ug) from murine lung or spleen.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
Concentration~0.25mg/ml
FormSupplied as a liquid in PBS, 0.02% sodium azide, before the addition of 40% glycerol.
PurityPurified by affinity chromatography.
ImmunogenPeptide corresponding to amino acids 314-329 of murine CAD (1).
SpecificityRecognizes CAD at 40kD.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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