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Specifications

Clone Host Grade Applications Monoclonal Mouse Ascites B IH IC IF

Cadherins are members of a multigene family of single chain glycoprotein receptors mediating Ca2+-dependent cell-cell adhesion(1,2,3). The N-terminal part of these molecules is exposed on the external cell surface and contains the putative homophilic binding sites. This is followed by a typical single transmembrane sequence and usually a cytoplasmic C-terminal tail which mediates interaction with the microfilament system
through molecules such as catenins, plakoglobin, vinculin, and alpha-actinin.
Catalog #	C0107-03B
Applications	Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilutions	Western Blot: 1:1000-1:2000
	Immunofluorescence: 1:500-1:1000 on cultured cells using methanol/acetone fixation
	Immunohistochemistry (Paraffin): 1:500-1:1000
	Immunohistochemistry: Frozen
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	8J460
Host	Mouse
Source	Chicken
Concentration	Not Determined
Form	Supplied as a liquid, 15mM sodium azide.
Purity	Ascites
Immunogen	Synthetic peptide corresponding to the C terminal amino acids of chicken N-Cadherin with an extra N terminal lysine residue (24aa) conjugated to glutaldegyde to KLH. Cellular Localization: Cell membrane
Specificity	Recognizes all cadherins at ~125-140kD; location is tissue dependent. Species Crossreactivity: human, mouse, rat, feline, chicken, bovine, canine, goat, guinea pig, hamster, porcine, psammoys (sand rat), rabbit, sheep and snake.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate names	NCAD