Anti -Cadherin 11 (OB-Cadherin, Osteoblast, CDH11)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
E B IP IH |
|
| Cadherins are a large family of Ca(2+)-dependent cell-cell adhesion molecules. Classical cadherins contain ve EC domains and a conserved cytoplasmic domain. Based on amino acid alignment, classical cadherins are divided into type I and II subgroups. Type I cadherins, including cadherins E (epithelial), N (neural), P (placental), and R (retinal) differ from type I cadherins in their speci c amino acid sequences. Type II cadherins include human cadherin-5, -6, -8, -11, and -12. Cadherin 11 (OB-Cadherin) is a marker of the loosely connected and migratory cellular elements of the mesenchyme and a strong expression has been noted in brain, spinal cord, bone marrow and bone cells, and is regulated in human endometrial glandular epithelial and stromal cells. A variant Cadherin 11 gene (variant form) encodes a truncated protein with an unusual cytoplasmic domain not present in other cadherins. The variant protein is generated by alternative splicing, utilizing a 179 bp intronic sequence. Recently, Cadherin 11 mRNA and protein were found to be expressed in most invasive breast cancer cell lines tested, but not in non-invasive cell lines, suggesting that cadherin-11 may be a marker for aggressive, invasive tumor subsets. | | | Catalog # | C0107-07 | | Applications | Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. | | Recommended Dilutions | Western Blot: 2-4ug/ml | | Immunoprecipitation: 5ug. Native, human; Not tested for mouse. | | Immunohistochemistry (Frozen): 5-10ug/ml. Incubate for 30-60 minutes at RT or overnight at 4°C. | | Optimal dilutions to be determined by the researcher. | | Positive Control | MDA.MB231 cells (human) and mouse L broblast lysates. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1,k | | Clone No | 3G41 | | Host | Mouse | | Source | Human | | Concentration | ~0.5mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide. | | Purity | Purified by Protein A affinity chromatography. | | Immunogen | Recombinant protein corresponding to an intracellular domain of human Cadherin 11. | | Specificity | Recognizes the ~110kD human Cadherin 11 protein. Crossreactivity with other cadherin family members has not been observed. Species Crossreactivity: mouse | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
External Links
|
