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Specifications

Clone Host Grade Applications Polyclonal Rabbit Purified IH

Cadherins are a family of CA2+ dependent intercellular adhesion molecules that play a central role in controlling morphogenetic movements during development. Their function is regulated by association with the actin cytoskeleton by a complex of cytoplasmic proteins called the catenin (alpha, beta and gamma). Members of the cadherin family include Cadherin 6 (K-Cadherin), P-Cadherin, E-Cadherin (Uvomorulin), N-Cadherin (Neural Cadherin), R-Cadherin, Cadherin 5, L-CAM and EP-Cadherin.
Catalog #	C0107-10
Epitope	C-terminal
Positive Control	Tonsil, squamous epithelium
Applications	Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution	Immunohistochemistry: 2.5-5ug/ml for 30 min at RT. Formalin-fixed, paraffin-embedded. Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0. Stains human lung small cell carcinoma using peroxidase-conjugate and AEC chromogen. Note membrane staining of tumor cells.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (30-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Chicken
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.1% sodium azide. Also supplied without BSA and sodium azide. See C0107-10X.
Purity	Purified
Immunogen	Synthetic peptide from the C-terminal end of chicken N-cadherin.
Specificity	Recognizes members of the Cadherin family of proteins including E-cadherin, N-cadherin, P-cadherin and R-cadherin. Species crossreactivity: Human, mouse, rat, chicken, dog, monkey, cow and Xenopus.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.