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You are here:Home » Antibodies » Antibodies-Cadherin » Anti -Cadherin, pan

Anti -Cadherin, pan


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH
Cadherins are a multifunctional family of Ca2+ -dependent, transmembrane glycoproteins which promote and maintain cell adhesion in virtually all multicellular organisms. The cadherin superfamily comprises over forty proteins which are, on average, 50-60% homologous (1). Cadherin expression is required for the assembly of cells into solid tissues and importantly, cadherins are expressed in a tissue speci c fashion (2) . Homotypic cellular interactions are promoted by homophillic interactions between the extracellular regions of identical cadherin molecules on neighboring cells. Recent crystal structure analysis of an extracellular cadherin domain suggests that individual cadherin molecules cooperate to form a linear cell adhesion zipper (3) . In adherens junctions, cadherins are anchored to the actin cytoskeleton by interaction with the small cytoplasmic proteins b-catenin and g-catenin which both bind to the actin binding protein a-catenin (4,5) . The interaction of b-catenin with the cytoplasmic tail of cadherins and other cytoplasmic proteins, including Tcf-family transcription factors and the tumor suppressor protein APC, is thought to be mediated through a region of the b-catenin molecule containing multiple repeats of the 42 amino acid armadillo sequence motif (6). In addition to playing important roles in differentiation and tissue morphogenesis, cadherins also appear to play a signi cant role in modulating tumor invasion and metastasis (7). The expression of E-cadherin correlates inversely with the motile and invasive behavior of tumor cells. In addition, the tissue speci city of cadherin subtypes are becoming valuable markers for the identi cation and differential diagnosis of certain cancers (8,9).
Catalog #C0107-10A
ApplicationsSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry: 1-10ug/ml. For Formalin fixed, paraffin embedded sections, Heat Induced Epitope Retrieval (HIER) step is required prior to staining.
Western Blot: 1ug/ml
ELISA: 0.1-1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
FormSupplied as a liquid in PBS, pH 7.2, 0.1% sodium azide, before the addition of glycerol to 40%.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide derived from the C-terminus of the chicken N-cadherin protein.
SpecificityThis peptide antibody is broadly crossreactive with all members of the cadherin family of proteins including N-cadherin, E-cadherin, P-cadherin and R-cadherin. Rabbit anti-pan-Cadherin is useful as both a ubiquitous cadherin probe, as well as a marker for adherens junctions. Species crossreactivity: This antibody displays broad species crossreactivity including human, bovine, mouse, rat, chicken, amphibian (Xenopus), canine, monkey, as well as other species.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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