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You are here:Home » Antibodies » Abs to Cadherin » Anti -Cadherin, pan

Anti -Cadherin, pan

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH
Cadherins are calcium dependent cell adhesion molecules, which play an important role in the growth and development of cells via the mechanisms of control of tissue architecture and the maintenance of tissue integrity. In adhesion junctions, cadherins are bound to beta and gamma catenins, which in turn bind to alpha catenin, an actin binding protein. Cadherins play important role in tumor invasion and metastasis.
Catalog #C0107-10C
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 2-4ug/ml for 2 hours at RT.
Immunohistochemistry (Formalin-fixed, paraffin-embedded): 1:100 for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.
Positive ControlMCF-7 cells, tonsil or squamous epithelium.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceChicken
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, pH 7.6, 0.2% BSA, 15mM sodium azide, before the addition of glycerol to 40%.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide from the C-terminus of chicken N-Cadherin. Cellular Localization: Cell membrane.
SpecificityRecognizes the C-terminal of chicken Cadherin, pan at ~120kD. Species Crossreactivity: Human, mouse, rat, canine, monkey, bovine and Xenopus.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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