| Cadherins are calcium dependent cell adhesion molecules, which play important role in the growth and development of cells via the mechanisms of control of tissue architecture and the maintenance of tissue integrity. In adhesion junctions, cadherins are bound to beta and gamma catenins, which in turn bind to alpha catenin, an actin binding protein. Cadherins play important role in tumor invasion and metastasis. |
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| Catalog # | C0107-10F |
| Applications | Suitable for use in Immunohistochemistry. Other applications not tested. |
| Recommended Dilution | Immunohistochemistry: 1:100 for 10min at RT |
| Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10min followed by cooling at RT for 20min |
| Optimal dilutions to be determined by the researcher. |
| Positive Control | Tonsil, squamous epithelium |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Host | Rabbit |
| Source | Chicken |
| Concentration | ~0.1mg/ml |
| Form | Supplied as a liquid in PBS, 0.2% BSA, 0.05% sodium azide. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | Synthetic peptide corresponding to chicken N-cadherin, at C-terminal. |
| Specificity | Recognizes chicken Cadherin, pan. Species Crossreactivity: human, mouse, canine, monkey, bovine, xenopus and rat. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
