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You are here:Home » Antibodies » Abs to Cadherin » Anti -Cadherin E (Cadherin 1, E-Cadherin, L-CAM, Epithelial Cell Adhesion Molecule, Uvomorulin, CDH1)

Anti -Cadherin E (Cadherin 1, E-Cadherin, L-CAM, Epithelial Cell Adhesion Molecule,
Uvomorulin, CDH1)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH IC
Cadherins constitute a family of transmembrane glycoproteins involved in Ca2+-dependent cell-cell interactions. The members of this family are differentially expressed in various tissues. They function in the maintenance of tissue integrity and morphogenesis. Cadherins are divided into type I and type II subgroups. Type I cadherins include epithelial cadherin (E-cadherin, cadherin-1 or uvomorulin), neural cadherin (N-cadherin or cadherin-2), placental cadherin (P-cadherin or cadherin-3) and retinal cadherin (R-cadherin or cadherin-4). Kidney cadherin (K-cadherin or cadherin-6) and osteoblast cadherin (OB-cadherin or cadherin-11) are type II cadherins. One of teh best characterized cadherins is E-cadherin. It is a 120kD transmembrane glycoprotein consisting of an 80kD extracellular and a 40kD transmembrane and cytoplasmic part. The extracellular domains of E-cadherins are responsible for calcium binding which allows for homophilic interactions with other E-cadherin molecules on the same cell and neighboring cells. E-cadherin can interact heterophilically with integrin aeb7. The cytoplasmic domain of E-cadherin is linked to the actin cytoskeleton through the associated cytoplasmic catenin proteins. Establishing a complex localized to adherens junctions. In carcinomas, E-cadherin is frequently downregulated. This is consistent with its function of an invasion suppressor in normal epithelia.
Catalog #C0108-25B
ApplicationsSuitable for use in Flow Cytometry, Immunohistochemistry, Immunocytochemistry, and Western Blot, Inhibition effect on cell-cell adhesion. Other applications not tested.
Recommended DilutionImmunohistochemistry (Frozen): 1:50-1:100. Use ABC as detection method. Note: Use PBS, 0.1mM CaCl2 and 0.1mM MgCl2
Immunocytochemistry: Methanol fixation
Flow Cytometry: 1:50-1:100
Western Blot: 1:50-1:500
Optimum dilutions to be determined by researcher.
HybridomaNSO mouse myeloma cells with spleen cells from Balb/c mice.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No4A12
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. before the addition of glycerol to 40%. No stabilizing proteins added.
PurityPurified by Protein G affinity chromatography.
ImmunogenCadherin E (Cadherin 1, E-Cadherin, L-CAM, Epithelial Cell Adhesion Molecule, Uvomorulin, CDH1)
SpecificityRecognizes E-cadherin. Specificly reacts with human 120kD protein and its 80kD trypsin-resistant extracellular part.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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