| Several major families of cell adhesion molecules can be distinguished. These include the cadherins and N-CAMs, which play a crucial role in cell recognition and adhesion, while integrins are implicated in the adherence of cells to the extracellular matrix. |
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| Catalog # | C0108-25D |
| Cadherins constitute a family of proteins, the members of which are differentially expressed in the different tissues. One of the best characterized members is E-cadherin, which is prevalent in epithelial tissues. It has been shown to play a crucial role in the process of tumor cell metastasis. NCAM’s (neural cell adhesion molecules) exist in at least three forms of plasma membrane plycoproteins, which are expressed in a variety of tissues including most nerve cells. |
| The integrins, finally, form a large family of glycosylated transmembrane proteins that act as dimers of an a- and a beta-subunit in interconnecting the cytoskeleton and the extracellular matrix. |
| Applications | Suitable for use in Immunohistochemistry (frozen sections), Immunocytochemistry, and Western Blot. Other applications not tested. |
| Recommended Dilution | Immunohistochemistry: 1:25-1:100, with ABC as detection reagent |
| Western Blot: 1:50-1:500 |
| Note | Use PBS buffer containing 0.1mM calcium chloride and 0.1mM magnesium chloride. |
| Optimum dilutions to be determined by researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 4A14 |
| Host | Mouse |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. No stabilizing proteins added. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | Affinity purified 80 kD extracellular fragments of E-cadherin derived from tryptic digestion of A-431 human vulva carcinoma cells. |
| Specificity | Recognizes human Cadherin E. Specific reactivity with 120kD E-cadherin protein and its 80kD trypsin-resistant extracellular part. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
