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You are here:Home » Antibodies » Abs to Cadherin » Anti -Cadherin E (Cadherin 1, E-Cadherin, L-CAM, Epithelial Cell Adhesion Molecule, Uvomorulin, CDH1)

Anti -Cadherin E (Cadherin 1, E-Cadherin, L-CAM, Epithelial Cell Adhesion Molecule,
Uvomorulin, CDH1)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH IC
Several major families of cell adhesion molecules can be distinguished. These include the cadherins and N-CAMs, which play a crucial role in cell recognition and adhesion, while integrins are implicated in the adherence of cells to the extracellular matrix.
Catalog #C0108-25D
Cadherins constitute a family of proteins, the members of which are differentially expressed in the different tissues. One of the best characterized members is E-cadherin, which is prevalent in epithelial tissues. It has been shown to play a crucial role in the process of tumor cell metastasis. NCAMís (neural cell adhesion molecules) exist in at least three forms of plasma membrane plycoproteins, which are expressed in a variety of tissues including most nerve cells.
The integrins, finally, form a large family of glycosylated transmembrane proteins that act as dimers of an a- and a beta-subunit in interconnecting the cytoskeleton and the extracellular matrix.
ApplicationsSuitable for use in Immunohistochemistry (frozen sections), Immunocytochemistry, and Western Blot. Other applications not tested.
Recommended DilutionImmunohistochemistry: 1:25-1:100, with ABC as detection reagent
Western Blot: 1:50-1:500
Note Use PBS buffer containing 0.1mM calcium chloride and 0.1mM magnesium chloride.
Optimum dilutions to be determined by researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No4A14
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. No stabilizing proteins added.
PurityPurified by Protein G affinity chromatography.
ImmunogenAffinity purified 80 kD extracellular fragments of E-cadherin derived from tryptic digestion of A-431 human vulva carcinoma cells.
SpecificityRecognizes human Cadherin E. Specific reactivity with 120kD E-cadherin protein and its 80kD trypsin-resistant extracellular part.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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