Anti -Cadherin E (Cadherin 1, E-Cadherin, L-CAM, Epithelial Cell Adhesion Molecule,
Uvomorulin, CDH1)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rat |
Ascites |
B IP IF |
|
| E-cadherin (Uvomorulin, L-CAM, Epithelial Cell Adhesion Molecule, cell CAM 120/180) is a 123kD molecule of the cadherin family. One of the epithelial cell adhesion molecules, E-Cadherin, plays an important role in the formation of cell-cell contacts in epithelia irrespective their origin form ecto-, meso-or endodermal tissue. This early adhesion event between epithelial cells is a prerequisite for the assembly of all elements of the junctional complex. Furthermore, E-Cadherin plays a crucial role in the maintenance of the epithelial junctional complex and is as such an important molecule in maintaining epithelial integrity. Cadnerins constitute a familly of transmembrane glycoproteins involved in Ca2+ dependent cell-cell interactions. The members of this family are diferentially expressed in various tissues. One of the best characterized cadherins in E-cadherin, a 120 kD transmembrane glycoprotein consisting of an 80 kD extracellular and a 40kD transmembrane and cytoplasmic part. Cadherin E is a functional antibody which inhibits cell-cell adhesion. Antibodies against E-cadherin are suitable to characterize epithelial cells. E-Cadherin is involved in adhesion of epithelial cells (mostly homotypic and homophilic) and is expressed on immature erythroid cells. In addition, it blocks the interaction of E-cadherin molecules and therefore is suitable to study E-cadherin-mediated signal transduction. | | | Catalog # | C0108-25N | | Cadherins are a family of CA2+ dependent intercellular adhesion molecules that play a central role in controlling morphogenetic movements during development. Their function is regulated by association with the actin cytoskeleton by a complex of cytoplasmic proteins called the catenin (alpha, beta and gamma). Members of the cadherin family include Cadherin 6 (K-Cadherin), P-cadherin, E-cadherin (uvomorulin), N-cadherin (neural cadherin), R-Cadherin, cadherin 5, L-CAM and EP-Cadherin. | | Applications | Suitable for use in Immunofluorescence, Immunohistochemistry (frozen sections), Western Blot and Immunoprecipitation. Other applications not tested. | | Recommended Dilution | Immunofluorescence: 1:1600 | | Western Blot: 1:3000 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 8J1258 | | Host | Rat | | Source | Mouse | | Concentration | ~6mg/ml | | Form | Supplied as a liquid (ascites) containing 0.1% sodium azide. | | Purity | Ascites | | Immunogen | Mouse embryonal carcinoma cell line PCC4 Aza RI. | | Specificity | Recognizes mouse Cadherin E. Species Cross-reactivity: human, bovine, and canine. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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