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You are here:Home » Antibodies » Abs to Cadherin » Anti -CDH1, NT (E-Cadherin, Epithelial Cadherin, Uvomorulin, Cadherin-1, CAM 120/80, CD324, UVO) (Azide free) (HRP)

Anti -CDH1, NT (E-Cadherin, Epithelial Cadherin, Uvomorulin, Cadherin-1, CAM 120/80,
CD324, UVO) (Azide free) (HRP)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B FC
CDH1 is a classical cadherin from the cadherin superfamily. This protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization.
Catalog #C0108-25P-HRP
ApplicationsSuitable for use in Western Blot and Flow Cytometry. Other applications not tested.
Recommended DilutionsFlow Cytometry: 1:10-1:50
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.2. No preservative added (Azide free). Conjugated to HRP.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide corresponding to a region betwen aa160-189 from the N-terminal region of human CDH1, conjugated to KLH.
SpecificityRecognizes human CDH1 at ~97kD.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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