Anti -Cadherin E (Cadherin 1, E-Cadherin, L-CAM, Epithelial Cell Adhesion Molecule,
Uvomorulin, CDH1)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Highly Purified |
E B IH FC |
|
| One of the epithelial cell adhesion molecules, E-Cadherin, plays an important role in the formation of cell-cell contacts in epithelia irrespective their origin form ecto-, meso- or endodermal tissue. This early adhesion event between epithelial cells is a prerequisite for the assembly of all elements of the junctional complex. Furthermore, E-Cadherin plays a crucial role in the maintenance of the epithelial junctional complex and is as such an important molecule in maintaining epithelial integrity. Over 90% of the malignant tumors are carcimomas. One of the prerequisites for the release of carcinoma cells from the primary site might be a defect in intercellular adhesion mediated by the absence of E-Cadherin expression. Therefore, the expression of E-Cadherin might be an important parameter for the determination of the invasive potential of epithelial neoplasms, and for the transition of a benign to a malignant neoplasm. | | | Catalog # | C0108-31B | | Applications | | | Suitable for use in ELISA, Flow Cytometry, Western Blot, Immunohistochemistry and Inhibition. Other applications not tested. | | Recommended Dilution | | | ELISA: 2ug/ml | | Flow Cytometry: 1ug/ml | | Western Blot: 2ug/ml; reducing and non-reducing conditions | | Immunohistochemistry (Frozen, paraffin): 2ug/ml; Incubate overnight at 4°C. | | Inhibition: 1ug/ml inhibits E-cadherin-dependent cell-cell contact | | Hybridoma | P3X63-Ag8-U1 myeloma cells with spleen cells from Balb/c mice. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2a | | Clone No | 6D185 | | Host | Mouse | | Source | Human | | Concentration | ~2mg/ml | | Form | Supplied as a lyophilized powder from PBS, pH 7.4,1% BSA. No preservative added. Reconstitute in 50ul sterile ddH2O. Dilute further in 20mM TBS, pH 7.4, 10mM calcium chloride, 1% BSA. | | Purity | Purified by ammonium sulfate precipitation and ion exchange chromatography. | | Immunogen | Soluble E-cadherin from human placenta. | | Specificity | Recognizes human E-cadherin. Does not cross-react with rat E-cadherin. | | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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