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You are here:Home » Antibodies » Abs to Cadherin » Anti -Cadherin E (Cadherin 1, E-Cadherin, L-CAM, Epithelial Cell Adhesion Molecule, Uvomorulin, CDH1)

Anti -Cadherin E (Cadherin 1, E-Cadherin, L-CAM, Epithelial Cell Adhesion Molecule,
Uvomorulin, CDH1)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Highly Purified E B IH FC
One of the epithelial cell adhesion molecules, E-Cadherin, plays an important role in the formation of cell-cell contacts in epithelia irrespective their origin form ecto-, meso- or endodermal tissue. This early adhesion event between epithelial cells is a prerequisite for the assembly of all elements of the junctional complex. Furthermore, E-Cadherin plays a crucial role in the maintenance of the epithelial junctional complex and is as such an important molecule in maintaining epithelial integrity. Over 90% of the malignant tumors are carcimomas. One of the prerequisites for the release of carcinoma cells from the primary site might be a defect in intercellular adhesion mediated by the absence of E-Cadherin expression. Therefore, the expression of E-Cadherin might be an important parameter for the determination of the invasive potential of epithelial neoplasms, and for the transition of a benign to a malignant neoplasm.
Catalog #C0108-31B
Applications
Suitable for use in ELISA, Flow Cytometry, Western Blot, Immunohistochemistry and Inhibition. Other applications not tested.
Recommended Dilution
ELISA: 2ug/ml
Flow Cytometry: 1ug/ml
Western Blot: 2ug/ml; reducing and non-reducing conditions
Immunohistochemistry (Frozen, paraffin): 2ug/ml; Incubate overnight at 4°C.
Inhibition: 1ug/ml inhibits E-cadherin-dependent cell-cell contact
HybridomaP3X63-Ag8-U1 myeloma cells with spleen cells from Balb/c mice.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypeMonoclonal
IsotypeIgG2a
Clone No6D185
HostMouse
SourceHuman
Concentration~2mg/ml
FormSupplied as a lyophilized powder from PBS, pH 7.4,1% BSA. No preservative added. Reconstitute in 50ul sterile ddH2O. Dilute further in 20mM TBS, pH 7.4, 10mM calcium chloride, 1% BSA.
PurityPurified by ammonium sulfate precipitation and ion exchange chromatography.
ImmunogenSoluble E-cadherin from human placenta.
SpecificityRecognizes human E-cadherin. Does not cross-react with rat E-cadherin.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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