| The cadherin superfamily comprises over forty proteins which are ~50-60% homologous. Cadherin expression is required for the assembly of cells into solid tissues and importantly, cadherins are expressed in a tissue speci c fashion. Homotypic cellular interactions are promoted by homophillic interactions between the extracellular regions of like cadherin molecules on neighboring cells. Recent crystal structure analysis of an extracellular cadherin domain suggests that individual cadherin molecules cooperate to form a linear cell adhesion zipper. In addition to playing important roles in differentiation and tissue morphogenesis, cadherins also appear to play a significant role in modulating tumor invasion and metastasis. The expression of E-cadherin correlates inversely with the motile and invasive behavior of tumor cells. |
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| Catalog # | C0108-34 |
| Applications | Suitable for use in ELISA, Western Blot, Immunohistochemistry, Inhibition, Immunoprecipitation, Flow Cytometry and Immunofluorescence. Other applications not tested. |
| Recommended Dilutions | ELISA: 1:10,000 (immobilized A431 cells) |
| Western Blot: 1-10ug/ml |
| Immunohistochemistry (Frozen or FFPE): ~10ug/ml |
| Inhibition of E-cadherin-dependent cell-cell contact: ~200ug/ml |
| Immunoprecipitation: ~5ug/IP reaction |
| Flow Cytometry: ~10ug/ml |
| Immunofluoresence: ~2-5ug/ml |
| Optimal dilutions to be determined by the researcher. |
| Hybridoma | P3X63-Ag8-U1myeloma cells with spleen cells from Balb/c mice. |
| Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 3G42 |
| Host | Mouse |
| Source | Human |
| Concentration | ~2mg/ml (after reconstitution) |
| Form | Supplied as a lyophilized powder from PBS, 1% BSA. No preservatives added. Reconstitute with 50ul sterile ddH2O. Recommended Diluent: 20mM TBS, pH 7.4, 10mM calcium chloride, 0.1% sodium azide, 1% BSA. |
| Purity | Puri ed by ammonium sulfate precipitation and anion exchange chromatography. |
| Immunogen | Human breast tumor cell line MCF-7. |
| Specificity | Recognizes human epithelial cadherin (E-cadherin, uvomorulin). |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
