Anti -Cadherin E (Cadherin 1, E-Cadherin, L-CAM, Epithelial Cell Adhesion Molecule,
Uvomorulin, CDH1)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Purified |
IH |
|
| Cadherins are transmembrane glycoproteins involved in Ca++-dependent cell adhesion. The cadherin family, including E-Cadherin, N-Cadherin, P-Cadherin and R-Cadherin, plays an important role in tissue morphogenesis and structure. E- cadherin is a 120kD protein that mediates Ca++-dependent adhesion between epithelial cells and is involved in the generation of epithelial layers during development and the and maintenance of epithelial tissue in adults. The presence of E-cadherin has been reported in other cell types, including osteoblasts, peripheral nerve Schwann cells and Langerhans cells. The large number of papers linking E-cadherin, or lack of E-cadherin expression, with a broad range of cancers suggests that its immunodetection in tissue may be useful in the diagnosis and prognosis of a variety of cancers. Tumor analysis by immunohistochemical techniques revealed that the reduction and/or loss of E-cadherin expression in carcinomas (including tumors of the neck, prostate, esophagus and other cancers) correlates positively with tumor cell invasion and metastasis. Studies have also associated reduced expression of E-cadherin with invasiveness and poor prognosis in breast cancer and poor prognosis in bladder cancer. Other studies have indicated that analysis of E-cadherin and N-cadherin expression may be useful in differentiating pleural mesotheliomas (N-cadherin positive) and lung adenocarcinomas (E-cadherin positive). | | | Applications: | | | Suitable for use in Immunohistochemistry. Other applications not tested. | | | Catalog # | C0108-34E | | Recommended Dilutions | Immunohistochemistry (Formalin-fixed, paraffin-embedded or frozen sections): 1:50-1:200. Epitope retrieval required (Citrate Buffer, pH 6). | | Optimal dilutions to be determined by the researcher. | | Recommended Positive Control | Non-neural epithelia tissue | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1,k | | Clone No | 3G47 | | Host | Mouse | | Source | Human | | Concentration | ~0.05mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.1% sodium azide. | | Purity | Purified | | Immunogen | Recombinant protein corresponding to the cytoplasmic domain of human E-Cadherin. | | Specificity | Recognizes human Cahedrin E. Expected Staining Pattern: Mainly cellular membrane, some cytoplasmic. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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