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Specifications

Clone Host Grade Applications Monoclonal Mouse Purified E B IF

Cadherins are a family of transmembrane glycoproteins that play a key role in Calcium dependent cell-cell adhesion. Several members of the cadherin family have been identified so far, including E- (epithelial), P- (placental), N- (neuronal) and M- (muscle) cadherin. M-Cadherin is expressed in myoblasts and might be involved in skeletal muscle cell differentiation.
Catalog #	C0108-34Z1
Applications	Suitable for use in Immunofluorescence, ELISA, and Western Blot. Other applications not tested.
Recommended Dilution	Immunofluorescence: 5ug/ml
	ELISA: 0.05ug/ml
	Western Blot: 1ug/ml for AP/BCIP/NBT (MTT) detection; 0.5ug/ml for HRP/ECL detection
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Reconstitute with sterile ddH2O, sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	9L427
Host	Mouse
Source	Human
Concentration	As reported
Form	Supplied as a lyophilized powder from 2X PBS, PEG, sucrose, 0.09% sodium azide. Reconstitute with 1ml sterile, ddH2O.
Purity	Purified by thiophilic adsorption and size exclusion chromatography.
Immunogen	Recombinant extracellular domain of human M-Cadherin.
Specificity	Recognizes human Cadherin-M (Extracellular Domain). Does not cross-react with mouse.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.