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You are here:Home » Antibodies » Abs to Cadherin » Anti -Cadherin N (Cadherin 2, Neuronal, CDH2)

Anti -Cadherin N (Cadherin 2, Neuronal, CDH2)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified B
Cadherins are a family of CA2+ dependent intercellular adhesion molecules that play a central role in controlling morphogenetic movements during development. Their function is regulated by association with the actin cytoskeleton by a complex of cytoplasmic proteins called the catenin (alpha, beta and gamma). Members of the cadherin family include Cadherin 6 (K-Cadherin), P-cadherin, E-cadherin (uvomorulin), N-cadherin (neural cadherin), R-Cadherin, cadherin 5, L-CAM and EP-Cadherin.
N-Cadherin mRNA is found at elevated levels in brain and heart and at much lower level in liver. Mechanisms such as mRNA expression, cytokine modulation and protease mediated turnover modulate N-cadherin protein levels during development. In addition, N-cadherin function is indirectly regulated by endogeneous kinases and phosphatases. Tyrosine phosphorylation of Beta-Catenin complexed with N-Cadherin results in dissociation of N-Cadherin from actin. However, N-Cadherin also interacts with a PTP1-B-like phosphatase that dephosphorylates B-catenin and promotes N-Cadherin/actin association. This, N-Cadherin is an integral adhesion molecule whose function is regulated by protein-protein interactions and phosphorylation/dephosphorylation events.
Catalog #C0108-35
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 0.1-0.5ug/ml using total rat brain cells (2-3hours at RT or overnight at 4°C using ECL chemiluminescence detection). Major band at 130kD.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3D2
HostMouse
SourceMouse
Concentration~0.25mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 50% glycerol, 0.1% sodium azide.
PurityPurified
ImmunogenC-terminal region pepide corresponding to aa802-819 of mouse N-cadherin KPVGIRRLDERPIHAEPQ
SpecificityRecognizes human N-Cadherin.
Species CrossreactivityRat, mouse, and chicken.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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