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Anti -Cadherin N (Cadherin 2, Neuronal, CDH2)
Anti -Cadherin N (Cadherin 2, Neuronal, CDH2)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Purified |
B |
|
| Cadherins are a family of CA2+ dependent intercellular adhesion molecules that play a central role in controlling morphogenetic movements during development. Their function is regulated by association with the actin cytoskeleton by a complex of cytoplasmic proteins called the catenin (alpha, beta and gamma). Members of the cadherin family include Cadherin 6 (K-Cadherin), P-cadherin, E-cadherin (uvomorulin), N-cadherin (neural cadherin), R-Cadherin, cadherin 5, L-CAM and EP-Cadherin. | | | N-Cadherin mRNA is found at elevated levels in brain and heart and at much lower level in liver. Mechanisms such as mRNA expression, cytokine modulation and protease mediated turnover modulate N-cadherin protein levels during development. In addition, N-cadherin function is indirectly regulated by endogeneous kinases and phosphatases. Tyrosine phosphorylation of Beta-Catenin complexed with N-Cadherin results in dissociation of N-Cadherin from actin. However, N-Cadherin also interacts with a PTP1-B-like phosphatase that dephosphorylates B-catenin and promotes N-Cadherin/actin association. This, N-Cadherin is an integral adhesion molecule whose function is regulated by protein-protein interactions and phosphorylation/dephosphorylation events. | | | Catalog # | C0108-35 | | Applications | Suitable for use in Western Blot. Other applications not tested. | | Recommended Dilution | Western Blot: 0.1-0.5ug/ml using total rat brain cells (2-3hours at RT or overnight at 4°C using ECL chemiluminescence detection). Major band at 130kD. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 3D2 | | Host | Mouse | | Source | Mouse | | Concentration | ~0.25mg/ml | | Form | Supplied as a liquid in PBS, pH 7.2, 50% glycerol, 0.1% sodium azide. | | Purity | Purified | | Immunogen | C-terminal region pepide corresponding to aa802-819 of mouse N-cadherin KPVGIRRLDERPIHAEPQ | | Specificity | Recognizes human N-Cadherin. | | Species Crossreactivity | Rat, mouse, and chicken. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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