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You are here:Home » Antibodies » Antibodies-Cadherin » Anti -N-Cadherin

Anti -N-Cadherin


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Clone Host Grade Applications
Monoclonal Rat Highly Purified B IP IH
The cadherin superfamily of proteins are a group of calcium mediated cell-cell adhesion molecules. Cadherins are responsible for a whole range of processes including development, wound healing, cell-cell signaling, cell growth and differentiation. N-cadherin is found in many locations including cardiac adherins junctions, oral squamous epithelial cells, and breast epithelial cells. Studies have linked N-cadherin to cancer metastasis by showing the aggressive tumor cells had preferentially turned on N-cadherin as opposed to E- or P-cadherin.
Catalog #C0108-35G
ApplicationsSuitable for use in Immunohistochemistry, Western Blot, Inhibition, Immunoprecipitation, Affinity Chromatography, Epitope Mapping and Adhesion Inhibition. Other applications not tested.
Recommended DilutionImmunohistochemistry: 10ug/ml
Western Blot: 1-10ug/ml
Inhibition of N-cadherin-dependent cell-cell contact: 100ug/ml for adhesion blocking
Optimal dilutions to be determined by the researcher.
HybridomaP3X63-Ag8.U1 myeloma cells with spleen cells from Balb/c mice.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
Clone No3G48
FormSupplied as a lyophilized powder from PBS, 1% BSA. No preservatives added. Reconstitute with 50ul sterile ddH2O. Dilute further with PBS, 1% BSA, 0.1% sodium azide.
PurityPuri ed by ammonium sulfate precipitation and anion exchange chromatography.
ImmunogenNeuronal tissue of chick embryos
SpecificityRecognizse chicken N-cadherin. Species Crossreactivity: Xenopus
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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