Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Cadherin » Anti -N-Cadherin

Anti -N-Cadherin

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Monoclonal Rat Highly Purified B IP IH
The cadherin superfamily of proteins are a group of calcium mediated cell-cell adhesion molecules. Cadherins are responsible for a whole range of processes including development, wound healing, cell-cell signaling, cell growth and differentiation. N-cadherin is found in many locations including cardiac adherins junctions, oral squamous epithelial cells, and breast epithelial cells. Studies have linked N-cadherin to cancer metastasis by showing the aggressive tumor cells had preferentially turned on N-cadherin as opposed to E- or P-cadherin.
Catalog #C0108-35G
ApplicationsSuitable for use in Immunohistochemistry, Western Blot, Inhibition, Immunoprecipitation, Affinity Chromatography, Epitope Mapping and Adhesion Inhibition. Other applications not tested.
Recommended DilutionImmunohistochemistry: 10ug/ml
Western Blot: 1-10ug/ml
Inhibition of N-cadherin-dependent cell-cell contact: 100ug/ml for adhesion blocking
Optimal dilutions to be determined by the researcher.
HybridomaP3X63-Ag8.U1 myeloma cells with spleen cells from Balb/c mice.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No3G48
HostRat
SourceChicken
Concentration~2mg/ml
FormSupplied as a lyophilized powder from PBS, 1% BSA. No preservatives added. Reconstitute with 50ul sterile ddH2O. Dilute further with PBS, 1% BSA, 0.1% sodium azide.
PurityPuri ed by ammonium sulfate precipitation and anion exchange chromatography.
ImmunogenNeuronal tissue of chick embryos
SpecificityRecognizse chicken N-cadherin. Species Crossreactivity: Xenopus
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links