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Anti -Cadherin N (Cadherin 2, Neuronal, CDH2)
Anti -Cadherin N (Cadherin 2, Neuronal, CDH2)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
B IP IH IF |
|
| Cadherins are a multifunctional family of Ca2+-dependent, transmembrane glycoproteins which promote and maintain cell adhesion in virtually all multicellular organisms. The cadherin superfamily comprises over forty proteins which are, on average, 50-60% homologous (1). Cadherin expression is required for the assembly of cells into solid tissues and importantly, cadherins are expressed in a tissue speci c fashion (2). Homotypic cellular interactions are promoted by homophillic interactions between the extracellular regions of like cadherin molecules on neighboring cells. Recent crystal structure analysis of an extracellular cadherin domain suggests that individual cadherin molecules cooperate to form a linear cell adhesion zipper (3). In adherens junctions, cadherins are anchored to the actin cytoskeleton by interaction with the small cytoplasmic proteins beta-catenin and gamma catenin, which both bind to the actin binding protein alpha catenin (4,5). | | | Catalog # | C0108-35M | | Applications | Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Immuno uorescence: 1-3ug/ml | | Western Blot: 0.5-1ug/ml | | Immunoprecipitation: 3-5ug/IP reaction | | Immunohistochemistry: 1-5ug/ml (Formalin fixed, paraffin embedded tissue; Heat induced epitope retrieval required) | | Optimal dilutions to be determined by the researcher. | | Source | Ascites | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1,k | | Clone No | 3G49 | | Host | Mouse | | Source | Chicken | | Concentration | ~0.25mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide before the addition of glycerol to 40%. | | Purity | Purified by Protein A affinity chromatography. | | Immunogen | Recombinant protein representing a portion of the intracellular domain of chicken N-cadherin. | | Specificity | Recgnizes chicken N-cadherin. Does not crossreact with other cadherin family members including P- and E-cadherins. Species Crossreactivity by Western Blot | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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