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You are here:Home » Antibodies » Abs to Cadherin » Anti -Cadherin P (Cadherin 3, Placental, CDH3)

Anti -Cadherin P (Cadherin 3, Placental, CDH3)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit B IF
Cadherins are a superfamily of transmembrane glycoproteins that contain cadherin repeats of approximately 100 residues in their extracellular domain. Cadherins mediate calcium-dependent cell-cell adhesion and play critical roles in normal tissue development (1). The classic cadherin subfamily includes N-, P-, R-, Band E-cadherins as well as about ten other members which are found in adherens junctions (AJ), a cellular structure near the apical surface of polarized epithelial cells. The cytoplasmic domain of classical cadherins interacts with -catenin, -catenin (also called plakoglobin) and p120 catenin. -catenin and -catenin associate with -catenin, which links the cadherin-catenin complex to the actin cytoskeleton (1,2). Unlike and -catenin, p120 regulates cadherin adhesive activity and trafficking rather than having a structural role in the junctional complex (1-4). E-cadherin is considered an acting suppressor of invasion and growth of many epithelial cancers (1-3). Recent studies indicate that cancer cells have up-regulated N-cadherin in addition to loss of E-cadherin. This change in cadherin expression is called the "cadherin switch." N-Cadherin cooperates with the FGF receptor, leading to over-expression of MMP-9 and cellular invasion (3). In endothelial cells, VE-cadherin signaling, expression and localization are correlated with vascular permeability and tumor angiogenesis (5,6). Expression of P-cadherin, which is normally present in epithelial cells, is also altered in ovarian and other human cancers (7,8).
Catalog #C0108-37B
ApplicationsSuitable for use in Immunofluorescence and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
Immunofluorescence: 1:25
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG
Clone No8A8
HostRabbit
SourceHuman
FormSupplied in 10mM sodium HEPES, pH 7.5, 150 mM sodium chloride, 100ug/ml BSA, 50% glycerol, 0.02% sodium azide.
PurityAs reported
ImmunogenSynthetic peptide corresponding to residues near the carboxy terminus of human P-cadherin.
SpecificityRecognizes endogenous levels of total P-cadherin protein. This antibody does not cross-react with other cadherin family members.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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