Anti -Cadherin P (Cadherin 3, Placental, CDH3)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rabbit |
|
B IF |
|
| Cadherins are a superfamily of transmembrane glycoproteins that contain cadherin repeats of approximately 100 residues in their extracellular domain. Cadherins mediate calcium-dependent cell-cell adhesion and play critical roles in normal tissue development (1). The classic cadherin subfamily includes N-, P-, R-, Band E-cadherins as well as about ten other members which are found in adherens junctions (AJ), a cellular structure near the apical surface of polarized epithelial cells. The cytoplasmic domain of classical cadherins interacts with -catenin, -catenin (also called plakoglobin) and p120 catenin. -catenin and -catenin associate with -catenin, which links the cadherin-catenin complex to the actin cytoskeleton (1,2). Unlike and -catenin, p120 regulates cadherin adhesive activity and trafficking rather than having a structural role in the junctional complex (1-4). E-cadherin is considered an acting suppressor of invasion and growth of many epithelial cancers (1-3). Recent studies indicate that cancer cells have up-regulated N-cadherin in addition to loss of E-cadherin. This change in cadherin expression is called the "cadherin switch." N-Cadherin cooperates with the FGF receptor, leading to over-expression of MMP-9 and cellular invasion (3). In endothelial cells, VE-cadherin signaling, expression and localization are correlated with vascular permeability and tumor angiogenesis (5,6). Expression of P-cadherin, which is normally present in epithelial cells, is also altered in ovarian and other human cancers (7,8). | | | Catalog # | C0108-37B | | Applications | Suitable for use in Immunofluorescence and Western Blot. Other applications not tested. | | Recommended Dilution | Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. | | Immunofluorescence: 1:25 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG | | Clone No | 8A8 | | Host | Rabbit | | Source | Human | | Form | Supplied in 10mM sodium HEPES, pH 7.5, 150 mM sodium chloride, 100ug/ml BSA, 50% glycerol, 0.02% sodium azide. | | Purity | As reported | | Immunogen | Synthetic peptide corresponding to residues near the carboxy terminus of human P-cadherin. | | Specificity | Recognizes endogenous levels of total P-cadherin protein. This antibody does not cross-react with other cadherin family members. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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