| Cadherin-13, also known as H-Cadherin and T-Cadherin, is a GPI-linked calcium dependent adhesion molecule that is predominantily expressed in heart and brain. Cadherin-13 negatively influences cell growth and is down regulated in some cancers. A 116 residue N-terminal propeptide is proteolytically removed to generate mature Cadherin-13. Human and mouse proCadherin-13 share 95% amino acid identity. |
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| Catalog # | C0108-50C |
| Applications | Suitable for use in Western Blot, Flow Cytometry and ELISA. Other applications not tested. |
| Recommended Dilution | Direct ELISA: 0.5-1ug/ml, the detection limit to detect recombinant human is ~7ng/well. |
| Western Blot: 1-2ug/ml, detects recombinant human: 5ng/lane under non-reducing coditions. Not recommended for use under reducing conditions. |
| Flow Cytometry: 25ug/ml using NCI-H460 human large lung cancer cells. Add 10ul of the diluted solution to 1-2.5 x10e5 cells in a total reaction volume < 200ul. The binding of unlabeled monoclonal antibodies may be visualized by adding 10ul of a 25ug/ml stock solution of a secondary developing reagent such as IgG (FITC) goat anti-rat. |
| Optimal dilutions to be determined by the researcher. |
| Flow Cytometry Positive Control | NCI-H460 human large cell lung cancer cells. |
| Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG2a |
| Clone No | 7H192 |
| Host | Mouse |
| Source | Human |
| Concentration | ~0.5mg/ml |
| Form | Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | Recombinant corresponding to aa23-692 of human Cadherin-13 expressed in NS0 cells (Accession # P55290.) |
| Specificity | Recognizes human Cadherin-13. Does not cross-react with recombinant human ECAD, recombinant human NCAD, recombinant human PCAD, recombinant human VECAD, recombinant human CAD-8, recombinant human CAD-11, recombinant human CAD-12 or recombinant human CAD-17. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
