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You are here:Home » Antibodies » Abs to Cadherin » Anti -Cadherin T (Truncated Cadherin, Cadherin 13, Heart-Cadherin)

Anti -Cadherin T (Truncated Cadherin, Cadherin 13, Heart-Cadherin)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified E B FC
Cadherin-13, also known as H-Cadherin and T-Cadherin, is a GPI-linked calcium dependent adhesion molecule that is predominantily expressed in heart and brain. Cadherin-13 negatively influences cell growth and is down regulated in some cancers. A 116 residue N-terminal propeptide is proteolytically removed to generate mature Cadherin-13. Human and mouse proCadherin-13 share 95% amino acid identity.
Catalog #C0108-50C
ApplicationsSuitable for use in Western Blot, Flow Cytometry and ELISA. Other applications not tested.
Recommended DilutionDirect ELISA: 0.5-1ug/ml, the detection limit to detect recombinant human is ~7ng/well.
Western Blot: 1-2ug/ml, detects recombinant human: 5ng/lane under non-reducing coditions. Not recommended for use under reducing conditions.
Flow Cytometry: 25ug/ml using NCI-H460 human large lung cancer cells. Add 10ul of the diluted solution to 1-2.5 x10e5 cells in a total reaction volume < 200ul. The binding of unlabeled monoclonal antibodies may be visualized by adding 10ul of a 25ug/ml stock solution of a secondary developing reagent such as IgG (FITC) goat anti-rat.
Optimal dilutions to be determined by the researcher.
Flow Cytometry Positive ControlNCI-H460 human large cell lung cancer cells.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypeMonoclonal
IsotypeIgG2a
Clone No7H192
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant corresponding to aa23-692 of human Cadherin-13 expressed in NS0 cells (Accession # P55290.)
SpecificityRecognizes human Cadherin-13. Does not cross-react with recombinant human ECAD, recombinant human NCAD, recombinant human PCAD, recombinant human VECAD, recombinant human CAD-8, recombinant human CAD-11, recombinant human CAD-12 or recombinant human CAD-17.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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