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You are here:Home » Molecular Biology » MB-Enzymes, Restriction » CaiI (AlwNI)

CaiI (AlwNI)

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Specifications

5'-C A G N N N^C T G-3'
3'-G T C^N N N G A C-5'
Catalog #C0112
Concentration 10u/ul
Source Comamonas acidovorans Lti 19021
Buffer 33mM Tris-acetate (pH 7.9), 10mM magnesium acetate, 66mM potassium acetate and 0.1mg/ml BSA. Incubate at 37°C.
Diluent Buffer 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol.
Storage Buffer 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% glycerol.
Overdigestion AssayNo detectable change in the specific fragmentation pattern is observed after 320-fold overdigestion (20u/ug lambda DNA x 16 hours) with CaiI.
Ligation/Recutting AssayAfter 50-fold overdigestion (3u/ug DNA x 17 hours) with CaiI, more than 95% of the DNA fragments can be ligated at a 5'-termini concentration of 0.3uM. More than 95% of these can be recut.
Labeled Oligonucleotide (LO) AssayNo detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10units of restriction endonuclease for 4 hours.
Methylation EffectsCaiI does not cut CAGNNCm5CTG. Blocked by overlapping Dcm methylation.
Stability during Prolonged IncubationA minimum of 0.2units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37°C.
Thermal InactivationEnzyme is inactivated by incubation at 65°C for 20min.
Digestion of Agarose-embedded DNAA minimum of 5units of enzyme is required for digestion of 1ug of agarose-embedded lambda DNA in 16 hours.
Number of Recognition Sites in DNA
Lambda41
PhiX1740
M13mp18/191
pBR3221
pUC18/191
pUC571
pTZ19R/U1
pBluescriptIIKS(-/+)1
pBluescriptIISK(-/+)1
pACYC1771
pACYC1843
Unit DefinitionOne unit is defined as the amount of enzyme required to digest 1ug of lambda DNA in 1 hour at 37°C in 50ul of assay buffer.


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