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You are here:Home » Antibodies » Abs to Neuroscience » Anti -Calbindin (D28K, Vitamin D-dependent Calcium-binding Protein, CALB1)

Anti -Calbindin (D28K, Vitamin D-dependent Calcium-binding Protein, CALB1)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH
Calbindin-D is a highly conserved 28 kD (261 aa) protein that belongs to a family of low molecular weight calcium-binding proteins (CaBPs). Calbindin-D has 4 active calcium binding domains. It plays a role in the regulation of calcium and calcium related signalling in neurons and during embryo development.
Catalog #C0113-16A
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 0.5-1.0ug/ml, has a predicted length of 261aa and a MW of 28kD
Immunohistochemistry (formalin fixed and paraffin embedded tissues) 1-2ug/ml
Optimal dilution determined by the researcher.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG
Clone No9G324
HostMouse
SourceBovine
ConcentrationAs reported
FormSupplied as a lyophilized powder from 1.2% sodium acetate, 2% BSA, 0.2% sodium azide. Reconstitute with sterile PBS to concentration of 0.1-1mg/ml.
PurityPurified by affinity chromatography.
ImmunogenBovine kidney Calbindin-D.
SpecificityRecognizes rat Calbindin. Reacs only with calbindin-D-28K and not with other family members such as calretinin and calbindin-D-9K. Species sequence homology: bovine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesCALB1_BOVIN, CALB1


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