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You are here:Home » Antibodies » Antibodies-Enzymes, Phosphatase » Anti -Calcineurin A (CnA, Protein Phosphatase 2B, A subunit)

Anti -Calcineurin A (CnA, Protein Phosphatase 2B, A subunit)


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Calcineurin is a Ca/calmodulin-dependent serine-threonine phosphatase that plays an important role in transducing Ca-dependent signals in a variety of cell types. Calcineurin has also been shown to have a profound influence on the properties of striated muscle cells, including cardiac muscle. Calcineurin (also known as CALNA or CALNA1, Calcineurin-alpha, Protein phosphatase 2B or PP2B) is the Ca+/calmodulin-regulated protein phosphatase, first detected in skeletal muscle and brain, has been found in from yeast to mammals. It is a heterodimers of two subunits: Calcineurin B/CnB, the 19-kda Ca+-binding and regulatory subunit, and Calcineurin A/CnA, ~61-kda catalytic subunit that is highly homologous with PP1 and PP2A. Multiple catalytic subunits of calcineurin are derived from at least 2 structural genes, type 1 (calcineurin A-alpha) and type 2 (calcineurin A-beta, CALNA2), each of which can produce additional alternatively spliced transcripts. CnB belongs to the family of EF-hand Ca-binding proteins. Both CnB and calmodulin are important for the activation of the phosphatase activity of calcineurin. Calcineurin controls the production of many cytokines including IL-2, TNF-alpha in the T-cell activation pathway. Calcineurin mediated dephosphorylation of the nuclear factor of activated T-cells (NF-AT) is required for NF-AT activation, nuclear translocation, and subsequent gene expression in T-cells. The immunosuppressive drugs, such as FK506, inhibit activation of NF-AT by calcineurin.
Catalog #C0114-06
Human calcineurin A-alpha (~60kD) and calcineurin B (~19kD) were co-expressed with yeast myristoyl-CoAprotein N-myrisoyltransferase and purified to >95%. Recombinant and purified Calcineurin is N-myristoylated on the CnB, as in the natural protein, resulting into biologically active protein. For western blot +ve control , calcineurin is supplied in SDS-PAGE sample buffer (reduced). Load ~10 ul/lane to visualize with antibodies.
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1-10ug/ml using ECL. B subunit is ~19kD.
ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
FormSupplied as a liquid in 100mM Tris, pH 7.5, 0.2% BSA, 0.05% sodium azide, 40% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenA 12-aa synthetic peptide from the N-terminus of human calcineurin A subunit (KLH).
SpecificityRecognizes human Calcineurin A. Does not recognize the B-subunit of calcineurin. Species sequence homology: bovine, porcine, rat and mouse 100%
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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