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You are here:Home » Antibodies » Abs to Enzymes, Phosphatase » Anti -Calcineurin A (CnA, Protein Phosphatase 2B, A subunit)

Anti -Calcineurin A (CnA, Protein Phosphatase 2B, A subunit)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Calcineurin is a Ca/calmodulin-dependent serine-threonine phosphatase that plays an important role in transducing Ca-dependent signals in a variety of cell types. Calcineurin has also been shown to have a profound influence on the properties of striated muscle cells, including cardiac muscle. Calcineurin (also known as CALNA or CALNA1, Calcineurin-alpha, Protein phosphatase 2B or PP2B) is the Ca+/calmodulin-regulated protein phosphatase, first detected in skeletal muscle and brain, has been found in from yeast to mammals. It is a heterodimers of two subunits: Calcineurin B/CnB, the 19-kda Ca+-binding and regulatory subunit, and Calcineurin A/CnA, ~61-kda catalytic subunit that is highly homologous with PP1 and PP2A. Multiple catalytic subunits of calcineurin are derived from at least 2 structural genes, type 1 (calcineurin A-alpha) and type 2 (calcineurin A-beta, CALNA2), each of which can produce additional alternatively spliced transcripts. CnB belongs to the family of EF-hand Ca-binding proteins. Both CnB and calmodulin are important for the activation of the phosphatase activity of calcineurin. Calcineurin controls the production of many cytokines including IL-2, TNF-alpha in the T-cell activation pathway. Calcineurin mediated dephosphorylation of the nuclear factor of activated T-cells (NF-AT) is required for NF-AT activation, nuclear translocation, and subsequent gene expression in T-cells. The immunosuppressive drugs, such as FK506, inhibit activation of NF-AT by calcineurin.
Catalog #C0114-06
Human calcineurin A-alpha (~60kD) and calcineurin B (~19kD) were co-expressed with yeast myristoyl-CoAprotein N-myrisoyltransferase and purified to >95%. Recombinant and purified Calcineurin is N-myristoylated on the CnB, as in the natural protein, resulting into biologically active protein. For western blot +ve control , calcineurin is supplied in SDS-PAGE sample buffer (reduced). Load ~10 ul/lane to visualize with antibodies.
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1-10ug/ml using ECL. B subunit is ~19kD.
ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~
FormSupplied as a liquid in 100mM Tris, pH 7.5, 0.2% BSA, 0.05% sodium azide, 40% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenA 12-aa synthetic peptide from the N-terminus of human calcineurin A subunit (KLH).
SpecificityRecognizes human Calcineurin A. Does not recognize the B-subunit of calcineurin. Species sequence homology: bovine, porcine, rat and mouse 100%
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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