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You are here:Home » Molecular Biology » MB-Enzymes, Phosphatase » Calcineurin A (CnA; Protein Phosphatase 2B, A subunit), Recombinant, Human

Calcineurin A (CnA; Protein Phosphatase 2B, A subunit), Recombinant, Human

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Specifications

Calcineurin is a Ca/calmodulin-dependent serine-threonine phosphatase that plays an important role in transducing Ca-dependent signals in a variety of cell types. Calcineurin has also been shown to have a profound influence on the properties of striated muscle cells, including cardiac muscle. Calcineurin (also known as CALNA or CALNA1, Calcineurin-alpha, Protein phosphatase 2B or PP2B) is the Ca+/calmodulin-regulated protein phosphatase, first detected in skeletal muscle and brain, has been found in from yeast to mammals. It is a heterodimers of two subunits: Calcineurin B/CnB, the 19-kda Ca+-binding and regulatory subunit, and Calcineurin A/CnA, ~61-kda catalytic subunit that is highly homologous with PP1 and PP2A. Multiple catalytic subunits of calcineurin are derived from at least 2 structural genes, type 1 (calcineurin A-alpha) and type 2 (calcineurin A-beta, CALNA2), each of which can produce additional alternatively spliced transcripts. CnB belongs to the family of EF-hand Ca-binding proteins. Both CnB and calmodulin are important for the activation of the phosphatase activity of calcineurin. Calcineurin controls the production of many cytokines including IL-2, TNF-alpha in the T-cell activation pathway. Calcineurin mediated dephosphorylation of the nuclear factor of activated T-cells (NF-AT) is required for NF-AT activation, nuclear translocation, and subsequent gene expression in T-cells. The immunosuppressive drugs, such as FK506, inhibit activation of NF-AT by calcineurin.
Catalog #C0114-08
ApplicationsSuitable for use in Western Blot. Not suitable for use in ELISA or other applications where native protein is required as this preparation is not biologically active. Other applications not tested.
Recommended DilutionWestern Blot: Heat once and load on gels (10ul/lane).
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
SourceHuman, recombinant expressed in yeast
PurityHighly purified ( 95%)
ConcentrationNot determined
FormSupplied as a liquid in denaturing SDS-PAGE sample buffer (reduced).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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