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You are here:Home » Antibodies » Abs to Calcitonin » Anti -Calcitonin Gene-Related Peptide (CGRP)

Anti -Calcitonin Gene-Related Peptide (CGRP)

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Specifications

Clone Host Grade Applications
Polyclonal Chicken Serum IH
Catalog #C0115-01G
Calcitonin gene-related peptide (CGRP) is present in C-cells of the thyroid and in central and peripheral nerves. CGRP has several important physiologic roles it is a potent vasodilator, and can affect the force and rate of heart beat; it can modulate acetylcholine receptor function at the neuromuscular junction; it has been demonstrated to block tolerance to morphine; and it can modulate antigen presentation in Langerhans cells in the skin.
ApplicationsSuitable for use in Immunohistochemistry. Other applications not tested.
Recommended DilutionsImmunohistochemistry: 1:100-1:500. Used on perfusion fixed tissue. Perfusion: 1) calcium-free Tyrode’s solution, 2) paraformaldehyde-picric acid fixative, and 3) 10% sucrose in PBS as a cryo-protectant. Desired tissues were dissected and stored overnight in 10% sucrose in PBS. Slide-mounted tissue sections were processed for indirect immunofluorescence. Slides were incubated with blocking buffer for 1 hour at RT. C0115-01G was diluted with blocking buffer. Blocking buffer was removed from slides and they were then incubated for 18-24 hours at 4ºC with diluted C0115-01G. Slides were rinsed 3x and then incubated with secondary antibody for 1 hour at RT. Slides were again rinsed 3x and coverslipped. Staining was examined using fluorescence microscopy.
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1906: IgY, Chicken Pab Rb x Ch
I1906-10: IgY, Chicken-HRP Rb x Ch
I1906-12: IgY, Chicken-FITC Rb x Ch
I1906-14: IgY, Chicken-AP Rb x Ch
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypePolyclonal
IsotypeIgY
HostChicken
SourceRat
ConcentrationNot Determined
FormSupplied as a liquid, 0.05% sodium azide.
PuritySerum
ImmunogenSynthetic peptide KDNFVPTNVGSEAF-NH2 corresponding to rat CGRP.
SpecificityRecognizes rat CGRP.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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