Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Calcitonin » Anti -Calcitonin Gene Related Peptide (CGRP)

Anti -Calcitonin Gene Related Peptide (CGRP)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum E B
Calcitonin leads to a lowering of plasma calcium and phosphate levels via the inhibition of bone resorption and excretion through the kidneys. Calcitonin is found in C cells of the normal and hyperplastic thyroid.
Catalog #C0115-02G
The calcitonin family of bioactive peptides comprises of calcitonin, amylin, two calcitonin-gene related peptides (CGRP1, and CGRP2) and adrenomedullin (ADM). Calcitonin is 32aa peptide found in the parafollicular "C" cells of the thyroid in mammals. It is also found in a number of non-mammals. It regulated the mineral (calcium and phosphate) balance. Calcitonin causes hypercalcemia by acting as an inhibitor of osteoclast induced bone resorption. CGRP is a 37-aa peptide produced by tissue specific processing of the calcitonin gene.
Calcitonin is the major product in the thyroid, whereas CGRP is the major product in neural tissues. CGRP is a potent cardiovascular agent. It has structural similarity with amylin. CGRP is found in two isoforms (CGRP-I and CGRP-II) that differs only by 3 amino acids. Amylin is a 37-aa peptide produced in the pancreatic beta-cell secretory granules and is co-released with insulin. Amylin also has CGRP-like effects on bone metabolism. Amylin has specific binding sites in the CNS and it may regulate gastric emptying and influence carbohydrate metabolism. Adrenomedullin (ADM) is a 52-aa hypotensive peptide. It has structural similarity with CGRP and amylin. ADM is produced in peripheral tissues, adrenal medulla, lung, and kidney. ADM has specific receptors on astrocytes and it is unregulated in ischaemia.
The calcitonin family peptides probably act through G-protein coupled membrane receptors. Recently, a homolog of calcitonin receptor, CRLR (calcitonin-receptor-like receptor human 461 aa; rat/mouse 463 aa) was identified. It is now shown that CRLR can function as either a CGRP receptor or an ADM receptor, depending upon which members of a new family of proteins called receptor activity modifying proteins (RAMP1-3) are expressed.
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure antibody using ECL.
ELISA: 1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity purified. Control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a lyophilized powder. Reconstitute with 100ul sterile PBS.
PuritySerum
Immunogen19aa peptide mapping to the C-terminal region of human CGRP (conjugated to KLH). This region is least conserved in other calcitonin. Species Sequence Homology: Canine (100%), bovine, sheep, rat, chicken, (92%), frog and porcine (85%). There are just 2 aa change between the human CGRP1 and CGRP2. No significant sequence homology is detected with amylin, calcitonin or adrenomedullin.
SpecificityThe human is quite conserved in various species: canine (100%), bovine, sheep, rat , chicken, (92%), frog and porcine (85%). There are just 2 aa change between the human CGRP1 and CGRP2. However, no significant sequence homology is detected with amylin, calcitonin or adrenomedullin. The control peptide, because of its small size (~2kD), is not suitable for Western. It should be used in ELISA, Dot Blot or to neutralize antibody and confirm specificity of antibody
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links