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Specifications

Clone Host Grade Applications Monoclonal Rabbit Supernatant IH

Calcitonin is a 32aa peptide which can be demonstrated in C cells of the normal and hyperplastic thyroid. Staining for calcitonin may be used for the identification of a spectrum of C cell proliferative abnormalities ranging from C cell hyperplasia to invasive tumors. Staining for calcitonin in medullary carcinoma of the thyroid produces a fine granular pattern in the cytoplasm. Amyloid deposits within the tumor may also exhibit varying degrees of calcitonin activity.
Catalog #	C0115-08B
Applications	Suitable for use in Immunohistochemistry. Other aplications not tested.
Recommended Dilutions	Immunohistochemistry (Formalin/paraffin): 1:200 for 30 minutes at RT. No special pretreatment is required for the immunohistochemical staining of formalin/paraffin tissues. Optimal dilutions to be determined by the researcher.
Recommended Positive Control	Thyroid or medullary carcinoma
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG
Clone No	5G25
Host	Rabbit
Source	Human
Concentration	Not Determined
Form	Supplied as a liquid, 15mM sodium azide.
Purity	Supernatant
Immunogen	Synthetic peptide corresponding to aa1-32 of human calcitonin.
Cellular Localization	Cytoplasmic.
Specificity	Recognizes human Calcitonin. Species Crossreactivity: monkey, mouse, rat, canine, equine, sheep, porcine.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.