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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified IH

Calcitonin is a 32aa peptide which can be demonstrated in C cells of the normal and hyperplastic thyroid. Staining for calcitonin may be used for the identification of a spectrum of C cell proliferative abnormalities ranging from C cell hyperplasia to invasive tumors. Staining for calcitonin in medullary carcinoma of the thyroid produces a fine granular pattern in the cytoplasm. Amyloid deposits within the tumor may also exhibit varying degrees of calcitonin activity.
Catalog #	C0115-10X
Applications	Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution	Immunohistochemistry: 1:300 for 10min at RT
	Staining of formalin-fixed tissues requires boiling tissues sections in 10mM citrate buffer, pH 6.0, for 10min followed by cooling at RT for 20min
	Optimal dilutions to be determined by the researcher.
Positive Control	Thyroid medullary carcinoma
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Host	Rabbit
Source	Human
Concentration	~0.1mg/ml
Form	Supplied as a liquid in PBS, 0.2% BSA, 0.05% sodium azide.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Synthetic peptide corresponding to 1-32aa from human calcitonin.
Specificity	Recognizes human calcitonin. Species Crossreactivity: mouse, rat, monkey, canine, equine, sheep and porcine.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.