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You are here:Home » Antibodies » Abs to Calcitonin » Anti -Calcitonin, Human

Anti -Calcitonin, Human

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified IH
Calcitonin is demonstrated in C cells of the normal and hyperplastic thyroid. Staining for calcitonin may be used for the identification of a spectrum of C cell proliferative abnormalities ranging from C cell hyperplasia to invasive tumors. Staining for calcitonin in medullary carcinoma of the thyroid produces a fine granular pattern in the cytoplasm. This antibody is excellent for staining of routine formalin-fixed, paraffin-embedded tissues.
Catalog #C0115-11A
ApplicationsSuitable for use in Immunhistochemistry. Other applications have not been tested.
Recommended DilutionImmunohistochemistry (Formalin/paraffin): 2.5-5.0ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min. ollowed by cooling at RT for 20 min.
Optimal dilution to be determined by the researcher
Positive ControlThyroid or medullary carcinoma
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See C0115-11AX
PurityPurified by Protein A affinity chromatography.
ImmunogenA synthetic peptide from human calcitonin.
SpecificityRecognizes human calcitonin. Species Crossreactivity: monkey, mouse, rat, canine, equine, sheep, porcine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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