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You are here:Home » Antibodies » Abs to Ion Channel » Anti -Calcium Transport Protein 1 (CaT-1, ECAC2)

Anti -Calcium Transport Protein 1 (CaT-1, ECAC2)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Catalog #C0120-10
ApplicationsSuitable for use in ELISA, Western Blotting. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL. The expected size of Cat-1 is ~83kD. However, posttranslational modification may alter its size depending upon the species and cell type.
ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceRat
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.4 and 0.1% sodium azide, 40% glycerol.
PurityAffinity purified over the control-peptide Sepharose
ImmunogenA 18-aa peptide sequence, mapping within cytoplasmic N-terminus of rat CaT-1/ECAC2 (1) was synthesized, coupled to KLH
SpecificityThe peptide is 100% conserved in rat, mouse and 94% in Cat-1/htrp8A and htrp8b proteins. No significant sequence homology is seen with CaT-2 or other proteins. Antibody reactivity in various species is not known. Control peptide, because of its low MW (<3kD), is not suitable for Western. It should be used for ELISA or antibody blocking experiments (use 5-10 ug control peptide per 1 ug of aff pure IgG or 1 ul antiserum) to confirm antibody specificity (see below).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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