| Calcium (Ca+2 or Ca) is the most abundant cation and it is required for many physiological activities such as bone formation and it acts as a second messenger in signal transduction. However only 1% of Ca is present in ionic form in biological fluids. Ca concentration is regulated by calcitropic hormones that act on bone, kidney, and intestine. Extracellular Ca+-levels are sensed and regulated by Calcium Sensing receptor (CASR). When Ca levels are limiting then it must be taken up by active, transcellular pathways comprising (1) Ca++ entry across apical membrane, (2) cytosolic transport of Ca++ across the cell form apical to basolateral membrane facilitated by a family of low mol wt Calcium binding proteins (CABPs) that include vitamin D3-dependent Ca++ binding proteins (calbindin-D9k, Calbindin-28k, Calretinin, Parvalbumin, S100, calmodulin) and finally (3) an active extrusion of Ca++ through basolateral membrane mediated by Ca++-ATPase and Na+-Ca++ exchangers (NCX). Ca++ absorption in intestine and its reabsorption in kidney are carried out by Ca++ Transport (CaT) proteins, CaT-1, CaT-2 or Epithelial Ca Channel (ECAC1/ECAC2/CaT-Like (CaT-L) proteins. |
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| Catalog # | C0120-20 |
| Applications | Suitable for use in ELISA, Western Blot, Antibody Blocking, Other applications not tested. |
| Recommended Dilution | Western Blot: 1:1000-1:5000. The expected size of Cat-1 is ~83kD. However, posttranslational modification may alter its size depending upon the species and cell type. |
| ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well. |
| Antibody Blocking: use 5-10ug control peptide per 1ul antiserum to confirm antibody specificity. |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Host | Rabbit |
| Source | Rat |
| Concentration | Not determined |
| Form | Supplied as a liquid in 40% glycerol. |
| Purity | Neat serum |
| Immunogen | A 18-aa peptide sequence, mapping within cytoplasmic N-terminus of rat CaT-1/ECAC2, coupled to KLH. |
| Specificity | The peptide is 100% conserved in rat, mouse and 94% in Cat-1/htrp8A and htrp8b proteins. No significant sequence homology is seen with CaT-2 or other proteins. Antibody reactivity in various species is not known. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
