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You are here:Home » Antibodies » Antibodies-Ion Channel » Anti -Calcium Transport Protein 1 (CaT-1, ECAC2)

Anti -Calcium Transport Protein 1 (CaT-1, ECAC2)


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Clone Host Grade Applications
Polyclonal Rabbit Serum E B
Calcium (Ca+2 or Ca) is the most abundant cation and it is required for many physiological activities such as bone formation and it acts as a second messenger in signal transduction. However only 1% of Ca is present in ionic form in biological fluids. Ca concentration is regulated by calcitropic hormones that act on bone, kidney, and intestine. Extracellular Ca+-levels are sensed and regulated by Calcium Sensing receptor (CASR). When Ca levels are limiting then it must be taken up by active, transcellular pathways comprising (1) Ca++ entry across apical membrane, (2) cytosolic transport of Ca++ across the cell form apical to basolateral membrane facilitated by a family of low mol wt Calcium binding proteins (CABPs) that include vitamin D3-dependent Ca++ binding proteins (calbindin-D9k, Calbindin-28k, Calretinin, Parvalbumin, S100, calmodulin) and finally (3) an active extrusion of Ca++ through basolateral membrane mediated by Ca++-ATPase and Na+-Ca++ exchangers (NCX). Ca++ absorption in intestine and its reabsorption in kidney are carried out by Ca++ Transport (CaT) proteins, CaT-1, CaT-2 or Epithelial Ca Channel (ECAC1/ECAC2/CaT-Like (CaT-L) proteins.
Catalog #C0120-20
ApplicationsSuitable for use in ELISA, Western Blot, Antibody Blocking, Other applications not tested.
Recommended DilutionWestern Blot: 1:1000-1:5000. The expected size of Cat-1 is ~83kD. However, posttranslational modification may alter its size depending upon the species and cell type.
ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well.
Antibody Blocking: use 5-10ug control peptide per 1ul antiserum to confirm antibody specificity.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
ConcentrationNot determined
FormSupplied as a liquid in 40% glycerol.
PurityNeat serum
ImmunogenA 18-aa peptide sequence, mapping within cytoplasmic N-terminus of rat CaT-1/ECAC2, coupled to KLH.
SpecificityThe peptide is 100% conserved in rat, mouse and 94% in Cat-1/htrp8A and htrp8b proteins. No significant sequence homology is seen with CaT-2 or other proteins. Antibody reactivity in various species is not known.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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