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You are here:Home » Antibodies » Abs to Caldesmon » Anti -Caldesmon

Anti -Caldesmon

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Specifications

Clone Host Grade Applications
Monoclonal Mouse E B IF
Catalog #C0132-14C
Cellular Localization Cytoplasmic
ApplicationsSuitable for use in RID, Immunofluorescence, ELISA and Western Blot. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Numbern/a
Clone TypeMonoclonal
IsotypeIgG1
Clone No8J480
HostMouse
SourceChicken
ConcentrationAs reported
Form0.1% Sodium Azide; Raw Ascites
PurityAscites
ImmunogenPurified chicken gizzard caldesmon. Epitope: The antibody is directed to an epitope near the carboxy-terminusCa2+Calmodulin and F-actin binding site of caldesmon.
SpecificityUsing immunoblot techniques Monoclonal anti-Caldesmon localizes both the high molecular weight form and the low molecular weight non-muscle form of caldesmon. Monoclonal anti-Caldesmon localizes the high molecular weight form of caldesmon in porcine stomach and turkey gizzard, and the low molecular weight non-muscle caldesmon in gerbil fibroma cells (CCL-146), normal rat kidney cells (NRK), human EJ and Hut-11 cells and chicken embryo fibroblast cells. Species Crossreactivity: Crossreacts with Human, Rat, Gerbil, porcine and Turkey.Not yet tested in other species.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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