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You are here:Home » Antibodies » Abs to Caldesmon » Anti -Caldesmon, smooth

Anti -Caldesmon, smooth

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH
Caldesmon is an actin and myosin binding protein. It plays a major role in the regulation of smooth muscle and non-muscle contractile events. At least 5 isoforms are known from alternate splicing. High molecular weight h-caldesmon (isoform 1) of 120-150kD is predominantly expressed in smooth muscles. Lower molecular weight caldesmon l-caldesmon (isoforms 2,3,4 and 5) of 61-70kD is more abundant in non-muscle tissues and cells. This antibody reacts specifically with h-caldesmon
Catalog #C0132-14F
ApplicationsSuitable for use in Immunohistochemistry and Western Blot . Other applications not tested.
Recommended DilutionWestern Blot: 1-2 ug/ml
Immunohistochemistry (formalin, paraffin): 2.0-4.0ug/ml.
Optimal dilution determined by the researcher.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No9G326
HostMouse
SourceHuman
ConcentrationAs reported
FormSupplied as a lyophilized powder from 1.2% sodium acetate, 2% BSA, 0.2% sodium azide. Reconstitute with sterile PBS to concentration of 0.1-1mg/ml.
PurityPurified by affinity chromatography.
ImmunogenHuman uterus smooth muscle extract.
SpecificityRecognizes human caldesmon. Species Crossreactivity: mouse; rat
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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