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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IH

Caldesmon is an actin and myosin binding protein. It plays a major role in the regulation of smooth muscle and non-muscle contractile events. At least 5 isoforms are known from alternate splicing. High molecular weight h-caldesmon (isoform 1) of 120-150kD is predominantly expressed in smooth muscles. Lower molecular weight caldesmon l-caldesmon (isoforms 2,3,4 and 5) of 61-70kD is more abundant in non-muscle tissues and cells. This antibody reacts specifically with h-caldesmon
Catalog #	C0132-14F
Applications	Suitable for use in Immunohistochemistry and Western Blot . Other applications not tested.
Recommended Dilution	Western Blot: 1-2 ug/ml
	Immunohistochemistry (formalin, paraffin): 2.0-4.0ug/ml.
	Optimal dilution determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Clone No	9G326
Host	Mouse
Source	Human
Concentration	As reported
Form	Supplied as a lyophilized powder from 1.2% sodium acetate, 2% BSA, 0.2% sodium azide. Reconstitute with sterile PBS to concentration of 0.1-1mg/ml.
Purity	Purified by affinity chromatography.
Immunogen	Human uterus smooth muscle extract.
Specificity	Recognizes human caldesmon. Species Crossreactivity: mouse; rat
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.