Anti -Caldesmon, phosphorylated (Ser759) (CDM, Caldesmon 1, Caldesmon-1, CALD1, H CAD,
H-CAD, L-CAD)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rabbit |
Supernatant |
B |
|
| Caldesmon is a smooth muscle and nonmuscle regulatory protein that interacts with actin, myosin, tropomyosin, and calmodulin (1,2). Smooth muscle caldesmon is an elongated molecule with a calmodulin, tropomyosin, and actin-binding region at the C-terminus and a | | | myosin-binding domain at the N-terminus (3). Caldesmon stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, | | | caldesmon inhibits the actomyosin ATPase by binding to F- actin. This inhibition is reduced by calcium-calmodulin and is promoted by tropomyosin (4).Phosphorylation of caldesmon | | | by extracellular signal-regulated kinase (ERK) and p38 mitogen-activated protein (MAP) kinases in smooth muscle are important for actin and tropomyosin binding, and actomyosin inhibitory activity.In intact vascular smooth muscle Caldesmon is phosphorylated by proline-directed | | | protein kinases, members of the MAP kinase family, suggesting that caldesmon phosphorylation by MAP kinase may modulate smooth muscle contraction (5). | | | | | | Applications: | | | Suitable for use in Western Blot. Other applications not tested. | | | Catalog # | C0132-14P | | Recommended Dilution | Western Blot: 1:1500-1:3000 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG | | Clone No | 9H307 | | Host | Rabbit | | Source | Human | | Concentration | As reported | | Form | Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA. | | Purity | Supernatant | | Immunogen | A phospho-specific peptide corresponding to residues surrounding Serine 759 of human Caldesmon. | | Specificity | Recognizes human Caldesmon when phosphorylated Ser759. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. | | Alternate names | Caldesmon 1 Isoform 1, Caldesmon 1 Isoform 2, Caldesmon 1 Isoform 3, Caldesmon 1 Isoform 4, Caldesmon 1 Isoform 5 |
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