Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Caldesmon » Anti -Caldesmon, LMW

Anti -Caldesmon, LMW

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH IF
Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction. Two closely related variants of human caldesmon have been identified which differ in their eletrophoretic mobility and cellular distribution. The h-caldesmon variant (120-150kD) is predominantly expressed in smooth muscle whereas l-caldesmon (70-80kD) is found in nonmuscle tissue and cells. Neither of the two variants has been detected in skeletal muscle.
Catalog #C1032-40
MW of Antigen 80kD (l-caldesmon)
Epitope Not determined
Applications and Recommended DilutionsImmunofluorescence
Western Blotting (Ab 1-2ug/ml for 2hrs at RT)
Immunohistochemistry (Formalin/paraffin) (Use Ab at 1-2ug/ml for 30 min at RT) * [Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.] The optimal dilution for a specific application should be determined by the investigator.
Positive Control Rh30 cells. Uterus
Cellular Localization Cytoplasmic
Storage and Stability May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at-20°C or colder. Aliquots are stable for at least 12 months at-20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No3F41
HostMouse
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See C1032-40X
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant caldesmon
SpecificityRecognizes Caldesmon, LMW . Species Crossreactivity: human.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links