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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IH IF

Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction. Two closely related variants of human caldesmon have been identified which differ in their eletrophoretic mobility and cellular distribution. The h-caldesmon variant (120-150kD) is predominantly expressed in smooth muscle whereas l-caldesmon (70-80kD) is found in nonmuscle tissue and cells. Neither of the two variants has been detected in skeletal muscle.
Catalog #	C1032-40X
MW of Antigen	80kD (l-caldesmon)
Epitope	Not determined
Applications and Recommended Dilutions	Immunofluorescence
	Western Blotting (Ab 1-2ug/ml for 2hrs at RT)
	Immunohistochemistry (Formalin/paraffin) (Use Ab at 1-2ug/ml for 30 min at RT) * [Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.] The optimal dilution for a specific application should be determined by the investigator.
Positive Control	Rh30 cells. Uterus
Cellular Localization	Cytoplasmic
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at-20°C or colder. Aliquots are stable for at least 12 months at-20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1,k
Clone No	3F41
Host	Mouse
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide. See C1032-40
Purity	Purified by Protein G affinity chromatography.
Immunogen	Recombinant caldesmon
Specificity	Recognizes Caldesmon, LMW . Species Crossreactivity: human.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.