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You are here:Home » Antibodies » Abs to Calcium Binding Proteins » Anti -Calgranulin A (S100A8, Calcium-binding Protein A8, CP-10, MRP8)

Anti -Calgranulin A (S100A8, Calcium-binding Protein A8, CP-10, MRP8)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IC
Mouse Calgranulin A, also known as CP-10, S100A8 and MRP8, is a 10kD member of the S100 family of calcium- binding proteins. S100A8 contains short sequential modules, including an N-terminal alpha-helix, a Ca++-binding EF-hand segment, a short central linker region, a second EF-hand segment and a C-terminal alpha-helix. S100A8 noncovalently heterodimerizes with S100A9. In the presence of Ca++, the heterodimers form heterotetramers. The dimeric complex is found both intracellularly and extracellularly. It binds to heparan sulfate and is chemotactic for PMNs and macrophages. The amino acid sequence of mouse S100A8 is 80% and 57% identical to that of rat and human S100A8, respectively.
Catalog #C1033-50E
ApplicationsSuitable for use in Western Blot, Immunocytochemistry and ELISA. Other applications have not been tested.
Recommended DilutionsWestern Blot: 0.1-0.2ug/ml. The detection limit is ~25ng/lane and 5ng/lane under non-reducing and reducing conditions, respectively.
Immunocytochemistry: 10ug/ml. Detects S100A8 in mouse NmuMG cells. Cells were fixed with PBS, 4% paraformaldehyde for 20 minutes at RT and blocked with PBS, 10% normal donkey serum, 0.1% Triton X-100, 1% BSA for 45 minutes at RT. After blocking, cells were incubated with diluted C1033-50E overnight at 4°C followed by Rhodamine Red-coupled anti-goat IgG at RT in the dark for one hour. Between each step, cells were washed with PBS, 0.1% BSA.
Direct ELISA: 0.5-1ug/ml. The detection limit is ~0.2ng/well.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceMouse
Concentration~0.2mg/ml
FormSupplied as a liquid in PBS, 5% trehalose.
PurityPurified by immunoaffinity chromatography.
ImmunogenRecombinant corresponding to aa1-89 of mouse S100A8 Calgranulin A expressed in E. coli (P27005).
SpecificityRecognizes mouse Calgranulin A (S100 calcium-binding Protein A8) at ~10kD. In direct ELISAs and Western Blots, shows < 5% crossreactivity with recombinant mouse S100A9, recombinant mouse S100A10 and recombinant human S100B.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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