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You are here:Home » Antibodies » Abs to Calmodulin » Anti -Calmodulin-dependent Protein Kinase Type 1 (CaM-KI, CaM kinase I alpha, CAMKI-alpha)

Anti -Calmodulin-dependent Protein Kinase Type 1 (CaM-KI, CaM kinase I alpha, CAMKI-alpha)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
CaM kinase I belongs to a calcium-triggered signalling cascade involved in trasncriptional regulation, hormone production, translational regulation, regulation of actin filament organisation and neurite outgrowth. CaM kinase I is involved in calcium-dependent activation of the ERK pathway. It phosphorylates proteins with the substrate consensus sequence [MVLIF}-x-R-x(2)-[ST]-x(3)-[MVLIF]. It is activated by calcium ions or calmodulin. It reaches its maximum catalytic activity when phosphorylated by CAMKK1 or CAMKK2. CaM kinase I has a length of 370 amino acids (unprocessed precursor) and a predicted molecular weight of 41.3KDa. It is a monomer that exists in many tissues and is located within the cytoplasm or nucleus. It belongs to the protein kinase superfamily, the CAMK Ser/Thr protein kinase family and the CaMK subfamily.
Catalog #C1035-03L
ApplicationsSuitable for use in Western Blot and ELISA. Other applications not tested.
Recommended DilutionWestern Blot: Denaturing conditions in HL-60 lysates
ELISA (Sandwich): Detection limit 0.3ng/ml with recombinant GST tagged CaM Kinase 1
Optimal dilution determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG
Clone No9G328
HostMouse
SourceHuman
Concentration~1mg/ml (after reconstitution)
FormSupplied as a lyophilized powder in PBS, pH 7.2. Reconstitute with 100ul sterile ddH2O.
PurityPurified by affinity chromatography.
ImmunogenPartial recombinant protein of human CaM kinase 1 (aa 271-370) with a GST tag.
SpecificityRecognizes human CaM Kinase 1 protein.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesCalcium/calmodulin-dependent protein kinase type 1, CaM-KI, CaM kinase I alpha, CAMKI-alpha, GENE NAME: CAMK1


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